Bai Yao-hui, Sun Qing-hua, Zhao Cui, Wen Dong-hui, Tang Xiao-yan
College of Environmental Sciences and Engineering,Peking University, Beijing 100871, China.
Huan Jing Ke Xue. 2010 Jul;31(7):1679-83.
In order to identify the characteristics of the plasmids of degrading bacterial strains and the relationship between the plasmids' function and biodegradation, plasmids were isolated from two bacterial strains (Paracoccus sp. BW001 and Shinella zoogloeoides BC026) and pulse-field gel electrophoresis was used to identify the distribution of plasmids and their molecular size. Two large plasmids with 190-245 kb and one small plasmid with 4.5-5.0 kb were found in the BW001, and at least 3 large plasmids over 200 kb were harbored in the BC026. The plasmid curing was conducted by high temperature-SDS method and the results indicated the biodegradation genes might locate in the plasmids of two bacterial strains. After transforming the plasmids of BW001 into E. coli 5alpha by electroporation, the new bacterial strain could tolerate pyridine.
为了鉴定降解菌株质粒的特征以及质粒功能与生物降解之间的关系，从两株细菌（副球菌属BW001和动胶菌属BC026）中分离质粒，并采用脉冲场凝胶电泳鉴定质粒的分布及其分子大小。在BW001中发现了两个大小为190 - 245 kb的大质粒和一个大小为4.5 - 5.0 kb的小质粒，而BC026中至少含有3个大小超过200 kb的大质粒。采用高温 - SDS法进行质粒消除，结果表明生物降解基因可能位于两株细菌的质粒中。通过电穿孔将BW001的质粒转入大肠杆菌5α后，新菌株能够耐受吡啶。
