School of Science, Western Sydney University, Penrith, NSW 2750, Australia.
Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh.
Molecules. 2023 Oct 1;28(19):6901. doi: 10.3390/molecules28196901.
Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily internalized by cells and, in combination with a green laser (λ = 532 nm) killed 94-98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm, and irradiance ~0.21 W/cm). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications.
癌症是主要的死亡原因之一，其治疗具有挑战性，包括高剂量要求、药物耐药性、传统化疗中肿瘤穿透性差和全身毒性。光动力疗法使用孟加拉玫瑰红（RB）等光敏剂和绿光激光，显示出对体外乳腺癌细胞的治疗潜力。然而，由于独特的临床微环境，亲水性 RB 难以有效地穿透肿瘤部位，在肿瘤部位聚集而不是进入癌细胞。在这项研究中，我们合成并表征了 RB 包封壳聚糖纳米颗粒，其峰值粒径约为 200nm。这些纳米颗粒很容易被细胞内化，与绿光激光（λ=532nm）联合使用，在低剂量（25μg/mL RB-纳米颗粒，辐照剂量126J/cm，辐照度0.21W/cm）下杀死了 94-98%的培养人乳腺癌细胞（MCF-7）和前列腺癌细胞（PC3）。此外，这些纳米颗粒对培养的人正常乳腺细胞（MCF10A）没有毒性，这为转化应用开辟了途径。
