采用罗氏 Cobas Amplicor 分枝杆菌结核检测试剂盒联合分枝杆菌属检测和核酸测序技术对临床标本进行分枝杆菌属的检测和鉴定。
Detection and identification of Mycobacterium spp. in clinical specimens by combining the Roche Cobas Amplicor Mycobacterium tuberculosis assay with Mycobacterium genus detection and nucleic acid sequencing.
机构信息
Institut für Medizinische Mikrobiologie, Nationales Zentrum für Mykobakterien, 8006 Zürich, Switzerland.
出版信息
J Clin Microbiol. 2010 Nov;48(11):3943-8. doi: 10.1128/JCM.00851-10. Epub 2010 Sep 8.
Abstract
We have recently developed a PCR assay for detection of Mycobacterium spp. at the genus level based on the Cobas Amplicor platform. The sensitivities for smear-positive and smear-negative specimens were found to be 100% and 47.9%, respectively. The specificity was 97.7%, the positive predictive value 84.6%, and the negative predictive value 93.1%. In a follow-up study, we have systematically evaluated the Mycobacterium genus assay in parallel with the Cobas Amplicor Mycobacterium tuberculosis assay on 2,169 clinical specimens, including respiratory and nonrespiratory specimens. Based on the genus assay, nontuberculous mycobacteria were readily detected and identified to the species level by PCR-mediated sequencing. In addition, our data point to a limited specificity of the Cobas Amplicor M. tuberculosis assay.
摘要
我们最近开发了一种基于 Cobas Amplicor 平台的聚合酶链反应（PCR）检测方法，用于检测分枝杆菌属水平的分枝杆菌属。结果显示，该方法对涂片阳性和涂片阴性标本的敏感性分别为 100%和 47.9%。特异性为 97.7%，阳性预测值为 84.6%，阴性预测值为 93.1%。在后续研究中，我们在 2169 例临床标本（包括呼吸道和非呼吸道标本）上，与 Cobas Amplicor 分枝杆菌结核检测方法平行系统地评估了分枝杆菌属检测方法。基于该检测方法，通过 PCR 介导的测序可快速检测并鉴定非结核分枝杆菌到种水平。此外，我们的数据表明 Cobas Amplicor 分枝杆菌结核检测方法的特异性有限。
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