实时 PCR 比传统 PCR 更适合用于临床样本中结核分枝杆菌复合群的检测吗？
Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?
机构信息
Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy.
出版信息
J Clin Microbiol. 2012 Aug;50(8):2810-3. doi: 10.1128/JCM.01412-12. Epub 2012 Jun 12.
Abstract
Cobas Amplicor MTB and later Cobas TaqMan MTB were used to test a very large series of consecutive specimens received for tuberculosis diagnosis. Performance parameters were estimated and compared overall and for separate specimen categories. Both systems showed excellent specificity, and that of TaqMan was the higher. The sensitivities were similar but satisfactory only with respiratory specimens and smear-positive samples.
摘要
Cobas Amplicor MTB 和后来的 Cobas TaqMan MTB 被用于检测大量连续的结核病诊断标本。对总体和单独的标本类别分别评估和比较了性能参数。两种系统均显示出极好的特异性，而 TaqMan 的特异性更高。灵敏度相似，但仅在呼吸道标本和涂阳标本中令人满意。
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