Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Stem Cells. 2010 Nov;28(11):2017-26. doi: 10.1002/stem.516.
The subventricular zone (SVZ) is the largest neurogenic region in the adult rodent brain. In the adult SVZ, unlike in the embryonic brain, neuronally committed precursor cells (neuroblasts) maintain their proliferative activity while migrating toward the olfactory bulb (OB), suggesting that they are inhibited from exiting the cell cycle. Little is known about the mechanisms underlying the unique ability of adult neuroblasts to proliferate during migration. Here, we studied the expression and function of Diversin, a component of the Wnt signaling pathways. In the neonatal and adult mouse brain, Diversin expression was observed in neuroblasts and mature neurons in the SVZ and hippocampus. Retrovirus-mediated overexpression of Diversin promoted the proliferation of neuroblasts and increased the number of neuroblasts that reached the OB. Conversely, the knockdown of Diversin decreased the proliferation of neuroblasts. Our results indicate that Diversin plays an important role in the proliferation of neuroblasts in the SVZ of the adult brain.
室下区（SVZ）是成年啮齿动物大脑中最大的神经发生区域。在成年 SVZ 中，与胚胎大脑不同，神经定向前体细胞（神经母细胞）在向嗅球（OB）迁移的同时保持其增殖活性，这表明它们被抑制退出细胞周期。关于成年神经母细胞在迁移过程中增殖的独特能力的机制知之甚少。在这里，我们研究了 Diversin 的表达和功能，它是 Wnt 信号通路的一个组成部分。在新生和成年小鼠的大脑中，Diversin 在 SVZ 和海马体中的神经母细胞和成熟神经元中表达。逆转录病毒介导的 Diversin 过表达促进了神经母细胞的增殖，并增加了到达 OB 的神经母细胞数量。相反，Diversin 的敲低减少了神经母细胞的增殖。我们的结果表明 Diversin 在成年大脑 SVZ 中神经母细胞的增殖中发挥重要作用。
