人黑素细胞中凝集素结合位点的超微结构定位

Ultrastructural localization of lectin binding sites in human melanocytes.

作者信息

Nau P, Schaumburg-Lever G

机构信息

Department of Dermatology, Eberhard-Karls-University Tübingen, Federal Republic of Germany.

出版信息

Arch Dermatol Res. 1990;282(8):520-5. doi: 10.1007/BF00371947.

DOI:10.1007/BF00371947

PMID:2082834

Abstract

The ultrastructural localization of carbohydrate residues in human melanocytes of normal epidermis and of one compound naevus was studied. The following lectins were used in a post-embedding technique: 1. peanut agglutinin (PNA), which reacts specifically with N-acetylgalactosamine; 2. Concanavalia ensiformis (Con A) indicating alpha-D-glucose and alpha-D-mannose binding sites; 3. Ulex europaeus agglutinin (UEA I) specific for alpha-L-fucose; 4. Wheat germ agglutinin (WGA), reacting specifically with N-acetyl-glucosamine and neuraminic acid (sialic acid); and 5. Limax flavus agglutinin (LFA), also specific for sialic acid (Neu5Ac-alpha-2,3-Gal and Neu5Ac-alpha-2,6-Gal). When incubated with WGA, Con A and LFA strong labelling was seen within the cytoplasm and in the plasma membrane of melanocytes, whereas incubations with PNA and UEA I revealed an occasional gold particle only. The determination of the distribution of carbohydrate residues in normal melanocytes is a prerequisite for future studies of abnormal melanocytes.

摘要

研究了正常表皮和一个复合痣的人黑素细胞中碳水化合物残基的超微结构定位。采用包埋后技术使用了以下凝集素：1. 花生凝集素（PNA），它与N-乙酰半乳糖胺特异性反应；2. 刀豆球蛋白A（Con A），指示α-D-葡萄糖和α-D-甘露糖结合位点；3. 欧洲荆豆凝集素（UEA I），对α-L-岩藻糖具有特异性；4. 麦胚凝集素（WGA），与N-乙酰葡糖胺和神经氨酸（唾液酸）特异性反应；5. 黄蛞蝓凝集素（LFA），也对唾液酸（Neu5Ac-α-2,3-Gal和Neu5Ac-α-2,6-Gal）具有特异性。当与WGA、Con A和LFA孵育时，在黑素细胞的细胞质和质膜内可见强烈标记，而与PNA和UEA I孵育时仅偶尔可见金颗粒。确定正常黑素细胞中碳水化合物残基的分布是未来对异常黑素细胞进行研究的前提条件。