Francoual J, Myara A, Trivin F, Leluc R, Odièvre M
Laboratoire de Biochimie, Hôpital Antoine-Béclère, Clamart.
Arch Fr Pediatr. 1990 Dec;47(10):721-3.
The assessment of reserve bilirubin binding capacity of serum in 12 children with type I Crigler-Najjar syndrome was performed by 3 biochemical methods. Two of them use chromatography techniques: thin layer on Sephadex or high performance liquid; they allow the determination of the saturation rate of all serum bilirubin carriers. The third method only evaluates the major carrier, albumin, using the molar bilirubin-albumin ratio. An identical correlation coefficient of 0.90 has been obtained when comparing with the chromatographic method on thin layer, respectively 20 determinations using high performance liquid chromatography and 122 measurements of the bilirubin/albumin ratio. In practice, we advise a first, simple and rapid determination of the bilirubin/albumin ratio in case of a sudden aggravation of the jaundice in the children with Crigler-Najjar type I disease. The chromatographic methods will be used in a second step, or in order to survey the phototherapy treatment that these children receive.
采用3种生化方法对12例Ⅰ型克里格勒-纳贾尔综合征患儿的血清胆红素储备结合能力进行了评估。其中两种方法采用色谱技术：葡聚糖凝胶薄层色谱法或高效液相色谱法；它们可以测定所有血清胆红素载体的饱和率。第三种方法仅使用胆红素-白蛋白摩尔比来评估主要载体白蛋白。分别与20次高效液相色谱测定和122次胆红素/白蛋白比值测量的薄层色谱法进行比较时，得到了相同的0.90相关系数。在实际操作中，对于Ⅰ型克里格勒-纳贾尔病患儿黄疸突然加重的情况，我们建议首先进行简单快速的胆红素/白蛋白比值测定。第二步将采用色谱法，或者用于监测这些患儿接受的光疗治疗。
