Department of Otolaryngology-Head and Neck Surgery, and Bao Zhang: Molecular Biology Laboratory, Southern Medical University, Guangzhou, Guangdong, PR China.
J Otolaryngol Head Neck Surg. 2010 Oct;39(5):511-5.
To investigate the expression of aquaporin-1 (AQP1) in nasopharyngeal tumour and nontumour tissues.
Thirty nasopharyngeal biopsy samples were collected and examined with both immunohistochemistry and real-time fluorescence quantitative polymerase chain reaction (RT-PCR) to detect the expression level of AQP1 in tissues.
Immunohistochemistry data suggested that AQP1 was expressed in both nasopharyngeal tumour and normal tissues, but tumour tissues were stained a lot stronger than nontumour tissues. RT-PCR analysis further confirmed the overexpression of AQP1 in tumour tissues; migrated tumour tissue had even higher expression.
The expression level of aquaporin-1 in nasopharyngeal cancer tissue could be involved in tumour migration.
研究水通道蛋白-1（AQP1）在鼻咽肿瘤组织和非肿瘤组织中的表达。
收集 30 例鼻咽活检组织，采用免疫组织化学和实时荧光定量聚合酶链反应（RT-PCR）检测 AQP1 在组织中的表达水平。
免疫组化数据表明，AQP1 在鼻咽肿瘤组织和正常组织中均有表达，但肿瘤组织的染色明显强于非肿瘤组织。RT-PCR 分析进一步证实了 AQP1 在肿瘤组织中的过度表达；转移的肿瘤组织表达更高。
水通道蛋白-1在鼻咽癌细胞中的表达水平可能与肿瘤迁移有关。
