Department of Molecular and Structural Biochemistry, North Carolina State University, NC 27695, USA.
FEBS Lett. 2010 Oct 8;584(19):4203-7. doi: 10.1016/j.febslet.2010.08.051. Epub 2010 Sep 7.
When a point-mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point-mutations, distant from an essential highly dynamic kinase recognition loop in the response regulator Spo0F, lock this loop in an active conformation. This 'conformational trapping' results in functionally hyperactive Spo0F. Consequently, point-mutations are seen to affect functionally critical motions both close to and far from the mutational site.
当蛋白质中的点突变引起功能变化时，最常见的情况是将这种变化归因于局部结构的扰动。在这里，我们表明，点突变，远离响应调节剂 Spo0F 中必需的高度动态激酶识别环，将该环锁定在活性构象中。这种“构象捕获”导致 Spo0F 功能超活跃。因此，点突变被认为会影响突变部位附近和远处的功能关键运动。
