School of Biotechnology, Department of Molecular Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden.
Trends Pharmacol Sci. 2010 Nov;31(11):528-35. doi: 10.1016/j.tips.2010.07.006. Epub 2010 Sep 7.
To transverse the plasma membrane and gain access to the cellular interior is one of the major obstacles for many novel pharmaceutical molecules. Since the late 1990s, cell-penetrating peptides (CPPs) have been utilized as transport vectors for a broad spectrum of 'biological cargoes', ranging from inert gold particles to multifaceted macromolecules such as proteins and plasmids. Numerous studies have shown that CPPs are efficient carriers for bioactive cargoes in vitro. However, even though CPPs are versatile transport vectors, this does not guarantee they can be developed into useful pharmaceutical molecules. Nevertheless, recent progress in the field has shown CPPs to be effective for in vivo delivery with retained biological activity of a wide variety of bioactive cargoes into virtually any mammalian tissue. This review will focus on recent developments and applications for CPP delivery and distribution in vivo.
穿越质膜进入细胞内部是许多新型药物分子面临的主要障碍之一。自 20 世纪 90 年代末以来，细胞穿透肽（CPPs）已被用作多种“生物有效载荷”的转运载体，范围从惰性金颗粒到蛋白质和质粒等复杂的大分子。大量研究表明，CPPs 是生物有效载荷在体外的有效载体。然而，即使 CPPs 是多功能的转运载体，这也不能保证它们可以开发成有用的药物分子。尽管如此，该领域的最新进展表明，CPPs 可有效地将各种生物有效载荷递送至体内，且保留其生物活性，几乎可将其递送至任何哺乳动物组织。本综述将重点介绍 CPP 体内递送至分布的最新进展和应用。
