Sreelatha S, Padma P R
Faculty of Science, NUS, Singapore. sree_latha04yahoo.om
Forsch Komplementmed. 2010;17(4):189-94. doi: 10.1159/000318606. Epub 2010 Jul 30.
The present study was designed to evaluate the efficacy of Moringa oleifera leaves against carbon tetrachloride (CCl(4))-treated liver slices in vitro.
The study evaluated the antioxidant properties of Moringa oleifera leaves against CCl(4)-induced oxidative damage in liver slices.
CCl(4) treatment significantly decreased the activities of antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione S-transferase and caused decreased glutathione content and increased the thiobarbituric acid-reacting substances (TBARS). Treatment with Moringa oleifera extract increased the activities of antioxidant enzymes and glutathione content and reduced the levels of TBARS significantly. Observed reduction in the level of lipid peroxides showed a decreased tendency of peroxidative damage.
We conclude that, under these experimental conditions, the leaf extracts effectively suppress CCl(4)-induced oxidative stress. Our findings provide evidence to demonstrate that the possible mechanism of this activity may be due to the strong antioxidant property of the leaves.
本研究旨在评估辣木叶对四氯化碳（CCl₄）处理的肝切片的体外疗效。
本研究评估了辣木叶对CCl₄诱导的肝切片氧化损伤的抗氧化特性。
CCl₄处理显著降低了超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽S-转移酶等抗氧化酶的活性，导致谷胱甘肽含量降低，并增加了硫代巴比妥酸反应物质（TBARS）。用辣木提取物处理可提高抗氧化酶的活性和谷胱甘肽含量，并显著降低TBARS水平。观察到脂质过氧化物水平的降低表明过氧化损伤的趋势降低。
我们得出结论，在这些实验条件下，叶提取物可有效抑制CCl₄诱导的氧化应激。我们的研究结果提供了证据，证明这种活性的可能机制可能是由于叶具有强大的抗氧化特性。
