Department of Physiology, University Colleges of Science, Technology, and Agriculture, University of Calcutta, Kolkata, West Bengal, India.
J Med Food. 2011 Oct;14(10):1167-72. doi: 10.1089/jmf.2010.1506. Epub 2011 Aug 23.
The present study evaluated the hepatoprotective effect of aqueous ethanolic Moringa oleifera leaf extract (MoLE) against radiation-induced oxidative stress, which is assessed in terms of inflammation and lipid peroxidation. Swiss albino mice were administered MoLE (300 mg/kg of body weight) for 15 consecutive days before exposing them to a single dose of 5 Gy of ⁶⁰Co γ-irradiation. Mice were sacrificed at 4 hours after irradiation. Liver was collected for immunoblotting and biochemical tests for the detection of markers of hepatic oxidative stress. Nuclear translocation of nuclear factor kappa B (NF-κB) and lipid peroxidation were augmented, whereas the superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and ferric reducing antioxidant power (FRAP) values were decreased by radiation exposure. Translocation of NF-κB from cytoplasm to nucleus and lipid peroxidation were found to be inhibited, whereas increases in SOD, CAT, GSH, and FRAP were observed in the mice treated with MoLE prior to irradiation. Therefore pretreatment with MoLE protected against γ-radiation-induced liver damage. The protection may be attributed to the free radical scavenging activity of MoLE, through which it can ameliorate radiation-induced oxidative stress.
本研究评估了水醇提取的辣木叶（MoLE）对辐射诱导的氧化应激的肝保护作用，其通过炎症和脂质过氧化来评估。将瑞士白化小鼠连续 15 天给予 MoLE（300mg/kg 体重），然后对其进行单次 5Gy ⁶⁰Co γ 射线照射。照射后 4 小时处死小鼠。收集肝脏进行免疫印迹和生化检测，以检测肝氧化应激的标志物。核因子 kappa B（NF-κB）的核易位和脂质过氧化作用增强，而超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）和铁还原抗氧化能力（FRAP）值则因辐射暴露而降低。MoLE 预处理可抑制 NF-κB 从细胞质向细胞核的易位和脂质过氧化作用，而在照射前用 MoLE 处理的小鼠中，SOD、CAT、GSH 和 FRAP 的增加则被观察到。因此，MoLE 预处理可防止 γ 射线诱导的肝损伤。这种保护作用可能归因于 MoLE 的自由基清除活性，它可以减轻辐射诱导的氧化应激。
