Bellayr Ian H, Gharaibeh Burhan, Huard Johnny, Li Yong
The Laboratory of Molecular Pathology, Stem Cell Research Center, Children's Hospital of UPMC, University of Pittsburgh, Pittsburgh, PA 15219, USA.
Int J Clin Exp Pathol. 2010 Mar 25;3(7):681-90.
The liver is unique for its ability to regenerate after injury, however, critical injuries or disease cause it to lose this quality. Stem cells have been explored as a possibility to restore the function of seriously damaged livers, based on their self-renewability and multiple differentiation capacity. These experiments examine the ability of muscle derived stem cells (MDSCs) to differentiate into hepatocyte-like cells in vitro and acquire functional liver attributes for repairing damaged livers. In vitro experiments were performed using MDSCs from postnatal mice and mouse hepatocyte cell lines. Our data revealed that MDSCs differentiated into hepatocyte-like cells and expressed liver cell markers, albumin, hepatocyte nuclear factor 4 α, and alpha feto-protein, both at the RNA and protein level. Additionally, in vivo studies showed successful engraftment of MDSCs into hepatectomized mouse livers of mice. These results provide evidence suggesting that MDSCs have the capacity to differentiate into liver cell-like cells and may serve as potential candidates to aid in liver regeneration.
肝脏因其在损伤后具有再生能力而独具特色，然而，严重损伤或疾病会使其丧失这种特性。基于干细胞的自我更新能力和多向分化能力，人们一直在探索将其作为恢复严重受损肝脏功能的一种可能性。这些实验研究了肌肉衍生干细胞（MDSCs）在体外分化为肝细胞样细胞并获得功能性肝脏属性以修复受损肝脏的能力。使用出生后小鼠的MDSCs和小鼠肝细胞系进行了体外实验。我们的数据显示，MDSCs在RNA和蛋白质水平上均分化为肝细胞样细胞，并表达肝细胞标志物、白蛋白、肝细胞核因子4α和甲胎蛋白。此外，体内研究表明MDSCs成功植入了肝切除小鼠的肝脏。这些结果提供了证据，表明MDSCs有能力分化为肝细胞样细胞，并可能成为有助于肝脏再生的潜在候选细胞。
