Department of General Surgery, Kocaeli University School of Medicine, Umuttepe, Kocaeli, Turkey,
Stem Cell Rev Rep. 2015 Apr;11(2):322-31. doi: 10.1007/s12015-014-9572-6.
In this study, we examined the effect of preoperatively administered resveratrol (RV) and mesenchymal stem cells (MSCs) on regeneration of partially hepatectomized rat liver. We also evaluated the effect of RV on homing of MSCs. MSCs were isolated from bone marrow and cultured in vitro. Wistar albino rats were randomly divided into four groups. In groups, rats received (1) no treatment, (2) single dose RV, (3) MSCs and (4) RV plus MSCs before partial hepatectomy (PH). Injected MSCs were traced by labeling them with green fluorescent protein, and liver regeneration was determined by comparison of liver weight gain, histological examination and immunohistochemical staining with proliferating cell nuclear antigen (PCNA) for mitotic cells. The expression levels of tumor necrosis factor -alpha (TNF-α), interleukin-6 (IL-6) and hepatocyte growth factor (HGF) were also determined in the parafin sections of liver specimens with immunohistochemical staining. Administration of RV and MSCs separately or together enhanced liver regeneration despite decreasing the TNF-α and IL-6 expression. This positive contribution was probably due to direct raising effect on HGF for RV and HGF expression for MSCs that we demonstrated with immunohistochemical staining. Additionally, RV increased the homing of MSCs in liver probably related to life prolonging effect on MSCs. These results indicate that preoperative RV as well as MSCs application enhances liver regeneration after partial hepatectomy in rats. Paying attention to RV about the effect on liver regeneration and homing of MSCs might be the goal of further investigations.
在这项研究中，我们研究了术前给予白藜芦醇（RV）和间充质干细胞（MSCs）对部分肝切除大鼠肝脏再生的影响。我们还评估了 RV 对 MSCs 归巢的影响。MSCs 从骨髓中分离出来并在体外培养。Wistar 白化大鼠随机分为四组。在组中，大鼠接受（1）无治疗，（2）单次 RV 剂量，（3）MSCs 和（4）RV 加 MSCs 在前部分肝切除（PH）之前。通过用绿色荧光蛋白标记注入的 MSCs 来追踪它们，通过比较肝重增加、组织学检查和增殖细胞核抗原（PCNA）免疫组织化学染色来确定肝再生，以检测有丝分裂细胞。还通过免疫组织化学染色检测肝组织石蜡切片中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和肝细胞生长因子（HGF）的表达水平。单独或联合使用 RV 和 MSCs 可增强肝再生，尽管降低了 TNF-α和 IL-6 的表达。这种积极的贡献可能是由于我们通过免疫组织化学染色证明的 RV 对 HGF 的直接升高作用和 MSCs 对 HGF 的表达。此外，RV 可能通过延长 MSCs 的寿命来增加 MSCs 在肝脏中的归巢。这些结果表明，术前 RV 以及 MSCs 的应用可增强大鼠部分肝切除后的肝再生。关注 RV 对肝再生和 MSCs 归巢的影响可能是进一步研究的目标。
