裸吻下口鲇（甲鲇科）微卫星标记的分离与特征分析

Isolation and characterization of microsatellite markers in the armored catfish Hypostomus gymnorhynchus (Loricariidae).

作者信息

Telles M P C, Resende L V, Brondani R P V, Collevatti R G, Costa M C, Silva Júnior N J

机构信息

Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.

出版信息

Genet Mol Res. 2010 Sep 8;9(3):1770-4. doi: 10.4238/vol9-3gmr868.

DOI:10.4238/vol9-3gmr868

PMID:20830668

Abstract

We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using these primers to analyze the polymorphism at each locus. All loci showed low polymorphism, with a low number of alleles per locus (1 or 2), except locus Hg_E19, which had 11 alleles. Expected heterozygosities for polymorphic loci ranged from 0.182 to 0.901. Combined paternity exclusion probability (0.857) was low and combined genetic identity (0.0026) was high, when we examined parentage. The low degree of polymorphism that we detected may be due to the small sample size and the small microsatellite size, despite the large motif size.

摘要

我们利用基因组鸟枪法文库获取重复序列，在披甲鲶鱼（Hypostomus gymnorhynchus，骨甲鲶科）中分离并鉴定了10个微卫星位点。设计了24对引物，并使用这些引物对来自巴西中部卡亚波河的14条裸吻下口鲶个体进行基因分型，以分析每个位点的多态性。除了具有11个等位基因的位点Hg_E19外，所有位点均显示出低多态性，每个位点的等位基因数量较少（1个或2个）。多态性位点的期望杂合度范围为0.182至0.901。在检查亲子关系时，组合父权排除概率（0.857）较低，而组合遗传同一性（0.0026）较高。尽管基序大小较大，但我们检测到的多态性程度较低可能是由于样本量小和微卫星大小小所致。