Gondim S G C A, Resende L V, Brondani R P V, Collevatti R G, Silva-Júnior N J, Pereira R R, Telles M P C
Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
Genet Mol Res. 2010 Aug 3;9(3):1513-7. doi: 10.4238/vol9-3gmr877.
We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable products using a single PCR protocol; six loci were polymorphic, but with a low number of alleles per locus (2 or 3). Expected heterozygosities for polymorphic loci ranged from 0.136 to 0.505. Combined paternity exclusion probability (0.638) was low and combined genetic identity (0.056) was high in studies of parentage. The low polymorphism may be due to the small microsatellite size and the large size of the motifs.
我们从一个基因组鸟枪法文库中鉴定出了狼鱼（Hoplias malabaricus，红鳍鱼科）的14个微卫星位点。设计了25对引物，并对来自巴西中部戈亚斯州西北部四个地点的48条马拉巴狼鱼个体进行基因分型，以表征每个位点的多态性。14对引物使用单一PCR方案扩增出了清晰可解释的产物；6个位点具有多态性，但每个位点的等位基因数量较少（2个或3个）。多态性位点的预期杂合度范围为0.136至0.505。在亲权研究中，组合父权排除概率（0.638）较低，组合遗传同一性（0.056）较高。低多态性可能是由于微卫星大小较小和基序大小较大所致。
