Kirst M, Cordeiro C M, Rezende G D S P, Grattapaglia D
The Plant Genetics Laboratory, EMBRAPA Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília 70770-970 DF, Brazil.
J Hered. 2005 Mar-Apr;96(2):161-6. doi: 10.1093/jhered/esi023. Epub 2004 Dec 15.
We report the genetic analysis of 192 unrelated individuals of an elite breeding population of Eucalyptus grandis (Hill ex Maiden) with a selected set of six highly polymorphic microsatellite markers developed for species of the genus Eucalyptus. A full characterization of this set of six loci was carried out generating allele frequency distributions that were used to estimate parameters of genetic information content of these loci, including expected heterozygosity, polymorphism information content (PIC), power of exclusion, and probability of identity. The number of detected alleles per locus ranged from 6 to 33, with an average of 19.8 +/- 9.2. The average expected heterozygosity was 0.86 +/- 0.11 and the average PIC was 0.83 +/- 0.16. Using only three loci, it was possible to discriminate all 192 individuals. The overall probability of identity considering all six EMBRA microsatellite markers combined was lower than 1 in 2 billion. An analysis of the sample size necessary to estimate expected heterozygosity with minimum variance indicated that at least 64 individuals have to be genotyped to characterize this parameter with adequate accuracy for most microsatellites in Eucalyptus. The high degree of multiallelism and the clear and simple codominant Mendelian inheritance of the set of microsatellites used provide an extremely powerful system for the unique identification of Eucalyptus individuals for fingerprinting purposes and parentage testing.
我们报告了对192个来自巨桉（Hill ex Maiden）优良育种群体的无亲缘关系个体进行的遗传分析，使用了为桉属物种开发的一组六个高度多态性微卫星标记。对这六个位点进行了全面表征，生成了等位基因频率分布，用于估计这些位点的遗传信息含量参数，包括预期杂合度、多态性信息含量（PIC）、排除能力和同一性概率。每个位点检测到的等位基因数量范围为6至33个，平均为19.8±9.2个。平均预期杂合度为0.86±0.11，平均PIC为0.83±0.16。仅使用三个位点就能够区分所有192个个体。考虑所有六个EMBRA微卫星标记组合后的总体同一性概率低于二十亿分之一。对以最小方差估计预期杂合度所需样本量的分析表明，对于大多数桉属微卫星，至少需要对64个个体进行基因分型，才能以足够的准确性表征该参数。所使用的微卫星组具有高度的多等位基因性以及清晰简单的共显性孟德尔遗传，为用于指纹识别目的和亲子鉴定的桉属个体的独特识别提供了一个极其强大的系统。
