Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, Germany.
Biomacromolecules. 2010 Oct 11;11(10):2818-23. doi: 10.1021/bm100910q.
Thin films of plasma polymerized pentafluorophenyl methacrylate (pp-PFM) offer highly reactive ester groups throughout the structure of the film that allow for subsequent reactions with different aminated reagents and biological molecules. The present paper follows on from previous work on the plasma deposition of pentafluorophenyl methacrylate (PFM) for optimum functional group retention (Francesch, L.; Borros, S.; Knoll, W.; Foerch, R. Langmuir 2007, 23, 3927) and reactivity in aqueous solution (Duque, L.; Queralto, N.; Francesch, L.; Bumbu, G. G.; Borros, S.; Berger, R.; Förch, R. Plasma Process. Polym. 2010, accepted for publication) to investigate the binding of a biologically active peptide known to induce cellular adhesion (IKVAV) and of biochemically active proteins such as BSA and fibrinogen. Analyses of the films and of the immobilization of the biomolecules were carried out using infrared reflection absorption spectroscopy (IRRAS), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). The attachment of the biomolecules on pulsed plasma polymerized pentafluorophenyl methacrylate was monitored using surface plasmon resonance spectroscopy (SPR). SPR analysis confirmed the presence of immobilized biomolecules on the plasma polymer and was used to determine the mass coverage of the peptide and proteins adsorbed onto the films. The combined analysis of the surfaces suggests the covalent binding of the peptide and proteins to the surface of the pp-PFM.
聚五氟苯甲基丙烯酸酯（pp-PFM）的等离子体聚合薄膜在整个膜结构中提供了高反应性的酯基，可与不同的胺化试剂和生物分子进行后续反应。本论文是在以前关于五氟苯甲基丙烯酸酯（PFM）等离子体沉积的研究基础上进行的，旨在最大限度地保留官能团（Francesch, L.; Borros, S.; Knoll, W.; Foerch, R. Langmuir 2007, 23, 3927）和提高在水溶液中的反应性（Duque, L.; Queralto, N.; Francesch, L.; Bumbu, G. G.; Borros, S.; Berger, R.; Förch, R. Plasma Process. Polym. 2010, accepted for publication），研究了一种已知能诱导细胞黏附的生物活性肽（IKVAV）以及一些具有生物化学活性的蛋白质（如 BSA 和纤维蛋白原）在薄膜上的结合情况。采用红外反射吸收光谱（IRRAS）、X 射线光电子能谱（XPS）和原子力显微镜（AFM）对薄膜和生物分子的固定化进行了分析。采用表面等离子体共振光谱（SPR）监测了生物分子在脉冲等离子体聚合五氟苯甲基丙烯酸酯上的附着情况。SPR 分析证实了生物分子固定在等离子体聚合物上，并用于确定吸附在薄膜上的肽和蛋白质的质量覆盖率。综合分析表明，肽和蛋白质与 pp-PFM 表面发生了共价结合。
