Laboratory of Nerve Regeneration, Department of Anatomy, Institute of Biology Laboratory of experimental gastroenterology, Department of Internal Medicine, University of Campinas, Campinas, Brazil.
Neuropathol Appl Neurobiol. 2010 Oct;36(6):515-34. doi: 10.1111/j.1365-2990.2010.01095.x.
Major histocompatibility complex (MHC) class I expression by neurones and glia constitutes an important pathway that regulates synaptic plasticity. The upregulation of MHC class I after treatment with interferon beta (IFN beta) accelerates the response to injury. Therefore the present work studied the regenerative outcome after peripheral nerve lesion and treatment with IFN beta, aiming at increasing MHC class I upregulation in the spinal cord.
C57BL/6J mice were subjected to unilateral sciatic nerve crush and treatment with IFN beta. The lumbar spinal cords were processed for immunohistochemistry, in situ hybridization, Western blotting and RT-PCR, while the sciatic nerves were submitted for immunohistochemistry, morphometry and counting of regenerated axons. Motor function recovery was monitored using the walking track test.
Increased MHC class I expression in the motor nucleus of IFN beta-treated animals was detected. In the peripheral nerve, IFN beta-treated animals showed increased S100, GAP-43 and p75NTR labelling coupled with a significantly greater number of regenerated axons. No significant differences were found in neurofilament or laminin labelling. The morphological findings, indicating improvements in the regenerative process after IFN treatment were in line with the motor behaviour test applied to the animals during the recovery process.
The present data reinforce the role of MHC class I upregulation in the response to injury, and suggest that IFN treatment may be beneficial to motor recovery after axotomy.
神经元和神经胶质细胞中主要组织相容性复合体（MHC）I 类的表达构成了调节突触可塑性的重要途径。干扰素β（IFNβ）治疗后 MHC I 类的上调加速了对损伤的反应。因此，本研究旨在通过增加脊髓中 MHC I 类的上调，研究周围神经损伤后和 IFNβ治疗的再生结果。
C57BL/6J 小鼠接受单侧坐骨神经挤压和 IFNβ治疗。对腰椎脊髓进行免疫组织化学、原位杂交、Western blot 和 RT-PCR 处理，同时对坐骨神经进行免疫组织化学、形态计量和再生轴突计数。使用行走轨迹测试监测运动功能恢复情况。
在 IFNβ处理动物的运动核中检测到 MHC I 类表达增加。在外周神经中，IFNβ处理动物的 S100、GAP-43 和 p75NTR 标记增加，再生轴突数量明显增加。神经丝或层粘连蛋白标记没有发现显著差异。形态学发现，表明 IFN 处理后再生过程的改善与应用于动物恢复过程中的运动行为测试一致。
本数据强化了 MHC I 类上调在损伤反应中的作用，并表明 IFN 治疗可能有益于轴突切断后的运动恢复。
