Zeng L, Jia L, Xu S, Yan Z, Ding S, Xu K
Department of Hematology, Affiliated Hospital of Xuzhou Medical College, PR China.
Transplant Proc. 2010 Sep;42(7):2720-4. doi: 10.1016/j.transproceed.2010.04.024.
Vascular endothelial injury, a feature of some complications of hematopoietic stem cell transplantation (HSCT), is characterized by increased endothelial cells. We investigated that classical pretreatment drugs in HSCT could result in vascular endothelial injury in mice. Six-to eight-week-old female BALB/c mice were divided into a control group, a cyclophosphamide group (60 mg/kg per day for 2 days) and a busulfan group (4 mg/kg per day for 4 days). We observed the general state of health and regularly counted the number of white blood cells. Circulating endothelial cells and their progenitors were estimated by flow cytometry. Morphologic endothelial changes were analyzed with optical and transmission electron microscopy. After conditioning with cyclophosphamide or busulfan, white blood cells fell to a low number with injuries noted on hematoxylin and eosin-stained pathology sections. Circulating endothelial cells and their progenitors peaked significantly higher than in the control group. Vascular endothelial injuries were observed in the 2 experimental groups by transmission electron microscopy. These data support the hypothesis the vascular endothelial injury occurs during conditioning with cyclophosphamide or busulfan for HSCT, with simultaneous mobilization of endothelial progenitor cells.
血管内皮损伤是造血干细胞移植（HSCT）某些并发症的一个特征，其特点是内皮细胞增多。我们研究了HSCT中经典的预处理药物是否会导致小鼠血管内皮损伤。将6至8周龄的雌性BALB/c小鼠分为对照组、环磷酰胺组（每天60mg/kg，共2天）和白消安组（每天4mg/kg，共4天）。我们观察了小鼠的一般健康状况，并定期计数白细胞数量。通过流式细胞术评估循环内皮细胞及其祖细胞。用光镜和透射电子显微镜分析内皮细胞的形态变化。经环磷酰胺或白消安预处理后，白细胞数量降至较低水平，苏木精-伊红染色病理切片显示有损伤。循环内皮细胞及其祖细胞的峰值显著高于对照组。透射电子显微镜观察到两个实验组均出现血管内皮损伤。这些数据支持这样的假说，即在HSCT使用环磷酰胺或白消安预处理期间会发生血管内皮损伤，同时内皮祖细胞被动员。
