Ben Amara Ibtissem, Troudi Afef, Soudani Nejla, Guermazi Fadhel, Zeghal Najiba
Animal Physiology Laboratory, Sfax Faculty of Science, BP 1171, 3000 Sfax, Tunisia.
Exp Toxicol Pathol. 2012 Mar;64(3):187-95. doi: 10.1016/j.etp.2010.08.005. Epub 2010 Sep 15.
Selenium has a pharmacological properties and it is well considered as an antioxidant. The present study investigated the potential ability of selenium, used as a nutritional supplement, to alleviate bone impairments in suckling rats whose mothers were treated with methimazole, an antithyroid drug.
Female Wistar rats were randomly divided into four groups of six each: group I served as control which received standard diet; group II were rendered hypothyroid by administration of methimazole (250 mg L(-1) in their drinking water); group III received both methimazole (250 mg L(-1) in their drinking water) and selenium (0.5 mg kg(-1) of diet); group IV received 0.5 Na(2)SeO(3) mg kg(-1) of diet. Treatments were started from the 14th day of pregnancy until day 14 after delivery.
Methimazole treatment decreased femur length and weight in 14-day-old rats, when compared to controls. Femur antioxidant enzyme activities, superoxide dismutase, catalase and glutathione peroxidase decreased. Lipid peroxidation recorded an increase revealed by high femur malondialdehyde levels. Methimazole also caused a significant decrease in calcium and phosphorus levels in bone. Yet, in plasma and urine, they increased and decreased inversely. Besides, plasma total tartrate-resistant acid phosphatase was enhanced, while total alkaline phosphatase was reduced. Co-administration of selenium through diet improved the biochemical parameters cited above. Nevertheless, distorted histoarchitecture revealed in hypothyroid rat femur was alleviated by Se treatment.
The present study suggests that selenium is an important protective element that may be used as a dietary supplement protecting against bone impairments.
硒具有药理特性，被公认为是一种抗氧化剂。本研究调查了作为营养补充剂的硒缓解哺乳期大鼠骨骼损伤的潜在能力，这些大鼠的母亲用抗甲状腺药物甲巯咪唑进行了治疗。
将雌性Wistar大鼠随机分为四组，每组六只：第一组作为对照组，给予标准饮食；第二组通过给予甲巯咪唑（饮用水中250 mg L(-1)）使其甲状腺功能减退；第三组同时接受甲巯咪唑（饮用水中250 mg L(-1)）和硒（饮食中0.5 mg kg(-1)）；第四组接受饮食中0.5 mg kg(-1)的亚硒酸钠。从怀孕第14天开始治疗，直至分娩后第14天。
与对照组相比，甲巯咪唑治疗使14日龄大鼠的股骨长度和重量降低。股骨抗氧化酶活性，即超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶降低。高股骨丙二醛水平显示脂质过氧化增加。甲巯咪唑还导致骨骼中钙和磷水平显著降低。然而，在血浆和尿液中，它们呈相反的增减。此外，血浆总抗酒石酸酸性磷酸酶增强，而总碱性磷酸酶降低。通过饮食共同给予硒改善了上述生化参数。尽管如此，硒治疗减轻了甲状腺功能减退大鼠股骨中扭曲的组织结构。
本研究表明，硒是一种重要的保护元素，可作为膳食补充剂用于预防骨骼损伤。
