Animal Physiology Laboratory, Faculty of Science, BP1171, 3000 Sfax, University of Sfax, Tunisia.
Hum Exp Toxicol. 2011 Oct;30(10):1549-60. doi: 10.1177/0960327110392403. Epub 2010 Dec 20.
The present study investigates the potential ability of selenium, considered as an antioxidant with pharmacological property to alleviate oxidative stress and hematological parameter disorders induced by methimazole, an antithyroid drug. Pregnant Wistar rats were randomly divided into four groups of six each: group I served as negative control and received a standard diet; group II received 250 mg/L of methimazole in drinking water and a standard diet; group III received both methimazole (250 mg/L, orally) and selenium (0.5 mg/kg of diet) supplemented to the standard diet; group IV served as positive control and received a supplement of selenium in the diet (0.5 mg/kg of diet) as sodium selenite (Na(2)SeO(3)). Treatment was started from the 14th day of pregnancy until day 14 after delivery. Methimazole reduced the number of red blood cells, hemoglobin concentration and hematocrit in mothers and their pups. Besides, plasma iron, vitamins B(9), B(12), C and E levels were reduced. Lipid peroxidation increased, objectified by high malondialdehyde levels and lactate dehydrogenase activity in plasma, while glutathione, glutathione peroxidase, superoxide dismutase and catalase activities showed a significant decline. Co-administration of selenium through diet improved all the parameters cited above. It can be concluded that the administration of selenium alleviates methimazole-induced toxicity, thus demonstrating its antioxidant efficacy.
本研究旨在探讨硒作为一种具有药理特性的抗氧化剂，是否具有缓解甲巯咪唑（一种抗甲状腺药物）引起的氧化应激和血液学参数紊乱的潜力。将妊娠 Wistar 大鼠随机分为四组，每组 6 只：第 I 组作为阴性对照，给予标准饮食；第 II 组给予 250mg/L 的甲巯咪唑饮用水和标准饮食；第 III 组给予甲巯咪唑（250mg/L，口服）和硒（饮食中 0.5mg/kg）补充剂；第 IV 组作为阳性对照，饮食中补充亚硒酸钠（Na2SeO3）作为硒（0.5mg/kg 饮食）。治疗从妊娠第 14 天开始，持续到分娩后第 14 天。甲巯咪唑降低了母亲及其幼崽的红细胞数量、血红蛋白浓度和血细胞比容。此外，血浆铁、维生素 B(9)、B(12)、C 和 E 水平降低。脂质过氧化增加，表现为血浆中丙二醛水平和乳酸脱氢酶活性升高，而谷胱甘肽、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶活性显著下降。通过饮食给予硒可以改善上述所有参数。可以得出结论，硒的给药缓解了甲巯咪唑引起的毒性，从而证明了其抗氧化功效。
