ESR Ltd., Kenepuru Science Centre, Porirua, New Zealand.
J Cyst Fibros. 2010 Dec;9(6):442-6. doi: 10.1016/j.jcf.2010.08.011. Epub 2010 Sep 15.
Burkholderia cepacia complex (Bcc) isolates from 39 CF patients and 25 non-CF patients in New Zealand were speciated and characterised using the multilocus sequence typing (MLST) scheme for Bcc. B. multivorans predominated in CF patients (31/39, 79.5%) and in non-CF patients (7/25, 28%). Sequence types (ST) with an international distribution were identified (27/64, 42.2%) among the New Zealand Bcc isolates. MLST revealed a high level of diversity among Bcc isolates in CF patients indicating a lack of person-to-person transmission. Non-CF patients showed less diversity in MLST types, however, individuals with shared STs were geographically and chronologically separated. The use of MLST analysis allows continued surveillance of isolates with the potential to identify outbreaks. The identification of internationally distributed strains may provide an indicator of the relative transmissibility and infectivity of these strains and warrants further investigation.
从新西兰的 39 名 CF 患者和 25 名非 CF 患者中分离出的伯克霍尔德氏菌复合群（Bcc）菌株，使用 Bcc 的多位点序列分型（MLST）方案进行了种系发生和特征描述。在 CF 患者（31/39，79.5%）和非 CF 患者（7/25，28%）中，B. multivorans 占优势。在新西兰的 Bcc 分离株中，确定了具有国际分布的序列型（ST）（27/64，42.2%）。MLST 显示 CF 患者的 Bcc 分离株具有高度多样性，表明人与人之间的传播不足。非 CF 患者的 MLST 类型多样性较低，但具有相同 ST 的个体在地理和时间上是分开的。MLST 分析的使用允许对具有潜在暴发风险的分离株进行持续监测。国际分布菌株的鉴定可能为这些菌株的相对传染性和感染性提供一个指标，并值得进一步研究。
