Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
Theriogenology. 2011 Jan 1;75(1):10-6. doi: 10.1016/j.theriogenology.2010.07.004. Epub 2010 Sep 15.
Lipid peroxidation (LPO) of dog spermatozoa was assessed in fresh semen and in samples of the same ejaculates after freezing and thawing. Particular attention was paid to individual differences in the susceptibility to LPO and its possible relationship with freezeability. Innate levels of LPO were low in fresh spermatozoa but increased after thawing in one of the dogs included in our study. The level of lipid peroxidation in fresh spermatozoa was not correlated with that of thawed spermatozoa. Negative correlations were detected between the activity in seminal plasma of GPx and sperm velocities post thaw (P < 0.01), however SOD activity was positively correlated with the percentage of linear motile sperm post thaw (P < 0.05).
检测了新鲜精液和经冷冻和解冻后的同一份精液样本中犬精子的脂质过氧化（LPO）。特别关注了 LPO 易感性的个体差异及其与可冻结性的可能关系。新鲜精子中的 LPO 先天水平较低，但在我们研究中的一只狗的解冻后增加。新鲜精子中的脂质过氧化水平与解冻后的精子水平无关。在解冻后（P<0.01），精液中 GPx 的活性与精子速度之间存在负相关，但 SOD 活性与解冻后线性运动精子的百分比呈正相关（P<0.05）。
