Khaki Amir, Araghi Atefeh, Lotfi Mehdi, Nourian Alireza
Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Iran Simmental Cattle Breeding Center, Amard-Dam Company, Amol, Iran.
Vet Res Forum. 2021 Winter;12(1):39-46. doi: 10.30466/vrf.2019.98781.2355. Epub 2021 Mar 15.
This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in the first and second ejaculations and their relationships with semen quality parameters in bulls. Repetitive ejaculates were separately collected, analyzed, and frozen from the sires. Progressive motility of frozen-thawed semen (PMFT) was considered the main factor for more data classification into three following groups: <40.00%, 40.00 - 50.00%, and >50.00%. Seminal plasma trace elements and enzymes were determined using atomic absorption spectrometry and ELISA, respectively. The results revealed significant differences between the first and second ejaculations. Semen concentration, SOD, GPx, and Fe were different in ejaculations. Although PMFT groups in different ejaculations did not show significant differences, there was significant alteration between different PMFT groups and first and second ejaculations. All frozen-thawed semen CASA parameters (except lateral head displacement) were associated with fresh motility parameters and before and after thawing sperm viability. Also, a correlation between seminal Zn concentration with fresh semen gross and progressive motility, average path velocity, and beat cross frequency, Cu with SOD and Fe and semen concentration was observed. CAT was associated with fresh and frozen-thawed sperm motility parameters except for lateral head displacement and angular displacement. Although our findings showed differences between the first and second ejaculations in some parameters, PMFT, which is the most important indicator for estimating bull fertility, was not different between them.
本研究旨在评估公牛首次射精和第二次射精时精浆中锌（Zn）、铜（Cu）、铁（Fe）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）水平的差异，以及这些差异与精液质量参数之间的关系。从种公牛采集重复射精样本，分别进行分析并冷冻保存。冷冻解冻精液的前向运动率（PMFT）被视为将更多数据分为以下三组的主要因素：<40.00%、40.00 - 50.00%和>50.00%。分别采用原子吸收光谱法和酶联免疫吸附测定法测定精浆中的微量元素和酶。结果显示首次射精和第二次射精之间存在显著差异。射精时精液浓度、SOD、GPx和Fe有所不同。虽然不同射精时的PMFT组未显示出显著差异，但不同PMFT组与首次射精和第二次射精之间存在显著变化。所有冷冻解冻精液的计算机辅助精液分析（CASA）参数（除头部侧向位移外）均与新鲜精液的运动参数以及解冻前后精子活力相关。此外，还观察到精浆锌浓度与新鲜精液的总运动率和前向运动率、平均路径速度以及鞭打交叉频率之间存在相关性，铜与SOD、铁和精液浓度之间存在相关性。CAT与新鲜和冷冻解冻精子的运动参数相关，头部侧向位移和角位移除外。虽然我们的研究结果显示在某些参数上首次射精和第二次射精之间存在差异，但作为评估公牛生育能力最重要指标的PMFT在两者之间并无差异。
