Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
Ecotoxicol Environ Saf. 2010 Nov;73(8):1875-83. doi: 10.1016/j.ecoenv.2010.08.012. Epub 2010 Sep 15.
This study investigated the acute and sub-lethal toxicities of waterborne mercuric chloride to Japanese flounder (Paralichthys olivaceus) embryos and larvae. Acute toxicity tests indicated that the 48-h LC(50) values of mercury to the embryos and larvae were 48.1 (32.8-63.6) and 99.4 (72.9-147.0) μg L(-1), respectively. Mercury could cause low hatching success, delayed hatching process, reduced growth at concentrations ≥20 μg L(-1), and induce reduced survival and higher morphological malformations at concentrations ≥40 μg L(-1) in the embryos and larvae. In sub-lethal toxicity test, superoxide dismutase (SOD) and catalase (CAT) activities, reduced glutathione (GSH) and malondialdehyde (MDA) contents of the larvae were significantly increased, while glutathione-S-transferase (GST) was decreased by 10 days of 0-10 μg Hg(2+)L(-1) exposure. These findings suggested that the hatching, survival, growth and antioxidant biomarkers of the flounder were sensitive to the highest mercury concentrations and could thereby serve as potential biomarkers for evaluating mercury contamination in the aquatic environment.
本研究调查了水合氯化汞对牙鲆（Paralichthys olivaceus）胚胎和幼体的急性和亚致死毒性。急性毒性试验表明，汞对胚胎和幼体的 48 小时半数致死浓度（LC50）值分别为 48.1（32.8-63.6）和 99.4（72.9-147.0）μg/L。在浓度≥20μg/L 时，汞会导致孵化成功率降低、孵化过程延迟、生长减缓，在浓度≥40μg/L 时，会导致胚胎和幼体的存活率降低和形态畸形增加。在亚致死毒性试验中，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、还原型谷胱甘肽（GSH）和丙二醛（MDA）含量在 0-10μg Hg2+/L 的暴露 10 天后显著增加，谷胱甘肽-S-转移酶（GST）则降低。这些发现表明，牙鲆的孵化、存活、生长和抗氧化生物标志物对最高汞浓度敏感，因此可以作为评估水环境污染中汞污染的潜在生物标志物。
