Department of Semen Biology and Gametes, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-747 Olsztyn, Poland.
Anim Reprod Sci. 2010 Dec;122(3-4):222-8. doi: 10.1016/j.anireprosci.2010.08.014. Epub 2010 Aug 22.
The aim of the study was to obtain baseline values for biochemical parameters of ostrich seminal plasma and sperm motility parameters measured by CASA. Biochemical characteristics of ostrich semen included a high protein concentration (29.3 ± 9.1g/l) and high amidase (280.6 ± 130.8 U/l) and LDH activity (1880.0 ± 983.6 U/l). On the other hand antioxidant, superoxide dismutase, anti-proteinase and acid phosphatase activity were low. Biochemical parameters of semen were variable. Motility of ostrich sperm was characterized by low linearity (23.0 ± 6.2%). The quality of undiluted semen stored at room temperature deteriorated within an hour due to agglutination and gelation. On the other hand, ostrich semen could be stored up to 4h at 5°C without loss of motility after which loss of motility occurred but could be partially mitigated using semen extenders (EK and Ovodyl).
本研究旨在获得鸵鸟精液生化参数和计算机辅助精子分析（CASA）测量的精子运动参数的基线值。鸵鸟精液的生化特征包括高蛋白浓度（29.3±9.1g/l）、高酰胺酶（280.6±130.8U/l）和 LDH 活性（1880.0±983.6U/l）。另一方面，抗氧化剂、超氧化物歧化酶、抗蛋白酶和酸性磷酸酶活性较低。精液的生化参数是可变的。鸵鸟精子的运动性特征是线性度低（23.0±6.2%）。在室温下，未经稀释的精液由于凝集和胶凝，在 1 小时内质量恶化。另一方面，鸵鸟精液可以在 5°C 下储存长达 4 小时，在使用精液稀释液（EK 和 Ovodyl）后不会损失运动能力，但会部分缓解。
