Ablamunits V G, Bliumkin V N, Mogilevskiĭ G M, Kirsanova L A, Baranova F S
Biull Eksp Biol Med. 1990 Dec;110(12):650-3.
Purified rat islets were dissociated into single-cell suspension with an EDTA-Trypsin treatment. During a stationary culture in vitro the islet cells reassociated forming aggregates (neoislets). Electron microscopy revealed that the aggregates consisted mostly of beta-cells and not numerous alpha-cells. They showed a good insulin-secreting capacity and were able to increase insulin release in response to glucose plus theophylline. The lack of passenger leukocytes makes the neoislets particularly suited for experimental and clinical transplantation.
纯化的大鼠胰岛经乙二胺四乙酸 - 胰蛋白酶处理后解离成单细胞悬液。在体外静止培养期间，胰岛细胞重新聚集形成聚集体（新胰岛）。电子显微镜显示，这些聚集体主要由β细胞组成，α细胞数量不多。它们表现出良好的胰岛素分泌能力，并且能够在葡萄糖加茶碱的刺激下增加胰岛素释放。缺乏过客白细胞使得新胰岛特别适合用于实验性和临床移植。
