Moriyoshi Kunihiko, Koma Daisuke, Yamanaka Hayato, Ohmoto Takashi, Sakai Kiyofumi
Environmental Technology Research Division, Osaka Municipal Technical Research Institute, Osaka, Japan.
Biosci Biotechnol Biochem. 2010;74(9):1940-2. doi: 10.1271/bbb.100213. Epub 2010 Sep 7.
An esterase gene from Neisseria sicca SB encoding CaeA, which catalyzes the deacetylation of cellulose acetate, was cloned. CaeA contained a putative catalytic domain of carbohydrate esterase family 1 and a carbohydrate-binding module (CBM) family 2. We constructed two derivatives, with and without the CBM of CaeA. Binding assay indicated that the CBM of CaeA had an affinity for cellulose.
克隆了来自干燥奈瑟菌SB的一个编码CaeA的酯酶基因，CaeA可催化醋酸纤维素的脱乙酰化反应。CaeA包含一个假定的碳水化合物酯酶家族1催化结构域和一个碳水化合物结合模块（CBM）家族2。我们构建了两个衍生物，一个带有CaeA的CBM，另一个没有。结合试验表明CaeA的CBM对纤维素具有亲和力。
