Włodarski K H
Department of Histology and Embryology, Medical Academy, Warszawa, Poland.
Folia Biol (Krakow). 1990;38(1-4):49-56.
The activity of alkaline and acid phosphatases in the bone marrow from the femoral cavity was investigated in the following groups of mice: (1) normal (non-irradiated); (2) irradiated with 600 R; (3) irradiated and repopulated with syngeneic bone marrow; (4) irradiated and repopulated with syngeneic marrow stroma; (5) non-irradiated, infused with allogeneic bone marrow (host versus graft reaction, HvG); (6) irradiated and repopulated with allogeneic bone marrow (graft versus host reaction, GvH). In addition, the activity of alkaline and acid phosphatases was examined in bone marrow stromal cultures. In irradiated animals the activity of both enzymes was lower than in non-irradiated ones, repopulation with syngeneic bone marrow restoring it to normal. Repopulation with allogeneic marrow (GvH) resulted in a very deep reduction of alkaline, but not acid, phosphatase. It is postulated that the decrease in bone marrow alkaline phosphatase activity can be a sensitive test for the early GvH reaction, preceding such parameters as splenomegaly. Marrow stroma cultured in vitro also showed very low alkaline phosphatase activity.
（1）正常（未照射）；（2）接受600伦琴照射；（3）照射后用同基因骨髓重新填充；（4）照射后用同基因骨髓基质重新填充；（5）未照射，输注异基因骨髓（宿主抗移植物反应，HvG）；（6）照射后用异基因骨髓重新填充（移植物抗宿主反应，GvH）。此外，还检测了骨髓基质培养物中碱性磷酸酶和酸性磷酸酶的活性。在受照射动物中，两种酶的活性均低于未受照射动物，用同基因骨髓重新填充可使其恢复正常。用异基因骨髓重新填充（GvH）导致碱性磷酸酶大幅降低，但酸性磷酸酶未降低。据推测，骨髓碱性磷酸酶活性降低可能是早期GvH反应的敏感检测指标，早于脾肿大等参数。体外培养的骨髓基质也显示出非常低的碱性磷酸酶活性。
