Su Qi-Jian, Zhou Ping, Bi Zhi-You, Xiao Xin, Wu Shu-Zhi, Cen Ping, Deng Wei, Liang Hao
Center for AIDS Research & School of Public Health, Guangxi Medical University, Nanning 530021, China.
Bing Du Xue Bao. 2010 Jul;26(4):290-4.
To investigate HIV-1 subtype distribution and prevalence of HIV-1 drug resistance in Liuzhou and Nanning, a total of 304 HIV-infected subjects or AIDS patients from Liuzhou and Nanning were recruited. Whole blood was withdrawn from a peripheral vein of each subject. HIV RNA were extracted from plasma, and subjected to PCR amplification targeting HIV pol gene fragment and DNA sequencing. Sequences obtained were subtyped by phylogenetic analysis. Two subtypes, CRF01_AE and CRF07_BC, were found in subjects from Liuzhou, accounting for 75.2% and 24.8%, respectively. Subtype CRF01 AE, CRFO8_BC, B, and C were found in subjects from Nanning. CRF01_AE and CRF08 BC were still the dominant strains in Nanning, accounting for 85.8% and 11.5%, respectively. Sequences were also analyzed for drug resistance mutations, and rates of drug resistance were calculated. The rate of drug resistance was 3.3% in ART-naive subjects from Liuzhou, and 8.7% in the ART-experienced. For patients from Nanning, the rate was 1.4% in ART-naive subjects, and 27.5% in ART-experienced subjects.
为调查柳州市和南宁市HIV-1亚型分布及HIV-1耐药性流行情况，共招募了304名来自柳州市和南宁市的HIV感染者或艾滋病患者。从每位受试者的外周静脉采集全血。从血浆中提取HIV RNA，并对HIV pol基因片段进行PCR扩增及DNA测序。通过系统发育分析对获得的序列进行亚型分型。在来自柳州市的受试者中发现了两种亚型，CRF01_AE和CRF07_BC，分别占75.2%和24.8%。在来自南宁市的受试者中发现了CRF01_AE、CRF08_BC、B和C亚型。CRF01_AE和CRF08_BC仍是南宁市的主要毒株，分别占85.8%和11.5%。还对序列进行了耐药性突变分析，并计算了耐药率。柳州市初治受试者的耐药率为3.3%，经治受试者为8.7%。对于南宁市的患者，初治受试者的耐药率为1.4%，经治受试者为27.5%。
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