[人类免疫缺陷病毒1型亚型及其在云南省的分布]

Yang Shao-min, Li Hui-qin, Chen Li-Li, Li Lin, Liu Yong-jian, Zhong Min, Li Jian-jian, Yang Bi-hui, Gao Li, Fan Yi-shan, Li Jing-yun

AIDS Care Center Laboratory, Yunnan Provincial Hospital of Infectious Disease, Kunming 650301, China.

Zhonghua Liu Xing Bing Xue Za Zhi. 2012 Aug;33(8):836-40.

OBJECTIVE

To understanding the genetic subtype and its population and regional distribution of HIV-1 strains circulating in Yunnan province.

METHODS

788 plasma specimens collected in 2008-2009 from 15 distracts of Yunnan, were enrolled. Viral RNA were extracted and subjected to RT-PCR. 1584 bp full length gag gene, 3147 bp full length pol gene and 558 bp env (C2V3) fragment were amplified and directly sequenced. Full length gag and pol genes were connected together as a complete genetic region (location on HXB2: 790 - 5096) for genotyping.

RESULTS

Of the 788 plasma specimens, a total number of 1728 genomic sequences including 599 gag, 564 pol and 525 env (C2V3) were successfully amplified and sequenced, with genotype of 617 samples identified. The subtypes of HIV-1 strains circulated in Yunnan were with the order of constituent ratio CRF08_BC (50.2%), CRF01_AE (25.0%), unknown recombinant forms (10.2%), CRF07_BC (9.2%), subtype C (2.9%) and subtype B (B') (2.4%). The distributions of subtypes showed significant regional differences and could be roughly divided into two forms:the CRF08_BC predominant areas represented by Lincang and Kunming, and the areas with CRF08_BC together with CRF01_AE coexistence, represented by Dehong and Xishuangbanna. The unknown recombinant forms accounted for more HIV infection in ethnic minorities (17.0%) than in ethnic Han (6.7%, P < 0.01). The distribution of subtypes varied significantly in the two primary routes of transmission for those infected through heterosexual contact. CRF08_BC and CRF01_AE were the dominant subtypes, accounting for 52.7% and 29.1% respectively. However, in IDUs, CRF08_BC strains accounted for half of the infection, while only 4.5% of the infections were caused by CRF01_AE, CRF07_BC while the unique recombinant forms were responsible for 15.5% infections. Of the 63 unknown recombinant forms, most (74.6%) were B (B') recombinant with C, while 25% were mosaic B and/or C fragments on the bases of CRF01_AE genome.

CONCLUSION

The subtypes of HIV-1 strains circulated in Yunnan were complicated under the significant differences of regions, ethnics or routes of transmission.

目的

了解云南省流行的HIV-1毒株的基因亚型及其人群和地区分布情况。

方法

纳入2008 - 2009年从云南省15个地区采集的788份血浆标本。提取病毒RNA并进行逆转录聚合酶链反应（RT-PCR）。扩增1584bp的gag基因全长、3147bp的pol基因全长以及558bp的env（C2V3）片段并直接测序。将gag和pol基因全长连接在一起作为一个完整的基因区域（在HXB2上的位置：790 - 5096）进行基因分型。

结果

在788份血浆标本中，成功扩增并测序了总共1728个基因组序列，包括599个gag、564个pol和525个env（C2V3），鉴定出617个样本的基因型。云南省流行的HIV-1毒株亚型按构成比依次为CRF08_BC（50.2%）、CRF01_AE（25.0%）、未知重组形式（10.2%）、CRF07_BC（9.2%）、C亚型（2.9%）和B（B'）亚型（2.4%）。亚型分布存在显著的地区差异，大致可分为两种形式：以临沧和昆明为代表的CRF08_BC为主的地区，以及以德宏和西双版纳为代表的CRF08_BC与CRF01_AE共存的地区。未知重组形式在少数民族中的HIV感染占比（17.0%）高于汉族（6.7%，P<0.01）。在异性传播感染的两种主要传播途径中，亚型分布差异显著。CRF08_BC和CRF01_AE是主要亚型，分别占52.7%和29.1%。然而，在注射吸毒者中，CRF08_BC毒株占感染的一半，而CRF01_AE仅导致4.5%的感染，CRF07_BC和独特重组形式导致15.5%的感染。在63种未知重组形式中，大多数（74.6%）是B（B'）与C的重组，而25%是基于CRF01_AE基因组的镶嵌B和/或C片段。