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亲本长末端重复序列对于构建稳定且高效复制的 HIV-1 CRF08_BC 型有感染性的分子克隆是很重要的。

Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.

机构信息

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

出版信息

PLoS One. 2012;7(2):e31233. doi: 10.1371/journal.pone.0031233. Epub 2012 Feb 17.

DOI:10.1371/journal.pone.0031233
PMID:22363589
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3281951/
Abstract

Circulating recombinant forms (CRFs) of HIV-1 have been identified in southern China in recent years. CRF08_BC is one of the most predominant subtypes circulating in China. In order to study HIV subtype biology and to provide a tool for biotechnological applications, the first full-length replication-competent infectious molecular clone harboring CRF08_BC is reported. The construction of this clone pBRGX indicates that a moderate-copy number vector is required for its amplification in E. coli. In addition, it is shown that the parental CRF08_BC LTRs are important for generating this efficient replication-competent infectious clone. These observations may aid in the construction of infectious clones from other subtypes. Both the pBRGX-derived virus and its parental isolate contain CCR5 tropism. Their full-length genomes were also sequenced, analyzed, compared and deposited in GenBank (JF719819 and JF719818, respectively). The availability of pBRGX as the first replication-competent molecular clone of CRF08_BC provides a useful tool for a wide range of studies of this newly emergent HIV subtype, including the development of HIV vaccine candidates, antiviral drug screening and drug resistance analysis.

摘要

近年来，中国南方已经发现了 HIV-1 的循环重组形式（CRFs）。CRF08_BC 是中国流行的主要亚型之一。为了研究 HIV 亚型生物学并为生物技术应用提供工具，报告了第一个携带 CRF08_BC 的全长复制型感染性分子克隆。该克隆 pBRGX 的构建表明，需要一个中等拷贝数的载体才能在大肠杆菌中扩增。此外，还表明亲本 CRF08_BC LTR 对于产生这种高效复制型感染性克隆是重要的。这些观察结果可能有助于从其他亚型构建感染性克隆。由 pBRGX 衍生的病毒及其亲本分离株都具有 CCR5 嗜性。它们的全长基因组也已测序、分析、比较并保存在 GenBank 中（分别为 JF719819 和 JF719818）。pBRGX 作为 CRF08_BC 的第一个复制型分子克隆，为广泛研究这种新出现的 HIV 亚型提供了有用的工具，包括 HIV 疫苗候选物的开发、抗病毒药物筛选和耐药性分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/e10adb8cf84a/pone.0031233.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/9c292ea2948e/pone.0031233.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/cdc214df6dba/pone.0031233.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/544b3265553c/pone.0031233.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/a68e97cbba3f/pone.0031233.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/0ec00c480afe/pone.0031233.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/ab257370a060/pone.0031233.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/e10adb8cf84a/pone.0031233.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/9c292ea2948e/pone.0031233.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/cdc214df6dba/pone.0031233.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/544b3265553c/pone.0031233.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/a68e97cbba3f/pone.0031233.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/0ec00c480afe/pone.0031233.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/ab257370a060/pone.0031233.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a658/3281951/e10adb8cf84a/pone.0031233.g007.jpg

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