Nonaka A, Manabe T, Kyogoku T, Tamura K, Tobe T
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Digestion. 1990;47(3):130-7. doi: 10.1159/000200487.
The role of free radicals in the development of cerulein-induced pancreatitis was evaluated by measuring the activity of the endogenous scavengers, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHpx), as indicators of the defense system, and the level of lipid peroxide (LPO) in the pancreas, as an indicator of the offense system. Acute pancreatitis was induced by 5 hourly intraperitoneal administrations of cerulein (50 micrograms/kg body weight), in 0.9% NaCl, to mice. The presence of acute pancreatitis was confirmed by changes in serum amylase levels and in typical microscopical features. Regarding the changes in the levels of endogenous scavengers, the SOD level was decreased significantly from a basal level of 52.6 +/- 3.94 to 43.1 +/- 2.79 mU/micrograms DNA at 6 h (p less than 0.01) to 38.8 +/- 5.18 mU/micrograms DNA at 9 h (p less than 0.05) and to 31.7 +/- 3.10 mU/micrograms DNA at 12 h (p less than 0.01) after the first intraperitoneal cerulein injection. The CAT level also decreased significantly from a basal level of 7.80 +/- 0.27 to 5.86 +/- 0.46 mU/micrograms DNA at 9 h (p less than 0.01) and to 4.52 +/- 0.21 mU/microgram DNA at 12 h (p less than 0.01). GSHpx increased from a basal level of 6.80 +/- 0.43 to 7.58 +/- 0.50 mU/micrograms DNA at 9 h and to 10.2 +/- 0.52 mU/micrograms DNA at 12 h after the first intraperitoneal cerulein injection.(ABSTRACT TRUNCATED AT 250 WORDS)
通过测量内源性清除剂超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSHpx）的活性作为防御系统的指标，以及胰腺中脂质过氧化物（LPO）的水平作为攻击系统的指标，来评估自由基在雨蛙肽诱导的胰腺炎发展过程中的作用。通过每5小时向小鼠腹腔注射雨蛙肽（50微克/千克体重），溶于0.9%氯化钠溶液中，诱导急性胰腺炎。通过血清淀粉酶水平的变化和典型的显微镜特征来确认急性胰腺炎的存在。关于内源性清除剂水平的变化，首次腹腔注射雨蛙肽后，SOD水平在6小时时从基础水平52.6±3.94显著降低至43.1±2.79毫单位/微克DNA（p<0.01），9小时时降至38.8±5.18毫单位/微克DNA（p<0.05），12小时时降至31.7±3.10毫单位/微克DNA（p<0.01）。CAT水平也从基础水平7.80±0.27显著降低至9小时时的5.86±0.46毫单位/微克DNA（p<0.01）和12小时时的4.52±0.21毫单位/微克DNA（p<0.01）。GSHpx在首次腹腔注射雨蛙肽后，从基础水平6.80±0.43在9小时时增加至7.58±0.50毫单位/微克DNA，12小时时增加至10.2±0.52毫单位/微克DNA。（摘要截选至250字）
