Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Caries Res. 2010;44(5):459-64. doi: 10.1159/000320267. Epub 2010 Sep 13.
The aim of this study was to determine the contribution of plaque and saliva towards the prolonged activity, also called substantivity, of three antimicrobial mouthrinses (Listerine®, Meridol®, Crest Pro Health®), used in combination with a toothpaste (Prodent Coolmint®). Volunteers brushed for 4 weeks with a toothpaste without antimicrobial claims, while during the last 2 weeks half of the volunteers used an antimicrobial mouthrinse in addition to brushing. At the end of the experimental period, plaque and saliva samples were collected 6 h after oral hygiene, and bacterial concentrations and viabilities were determined. The contribution of plaque and saliva towards substantivity was assessed by combining plaque obtained after mechanical cleaning only with plaque and saliva obtained after additional use of an antimicrobial rinse. Subsequently, resulting viabilities of the combined plaques were determined. The viabilities of plaque samples after additional rinsing with mouthrinses were lower than of plaque obtained after mechanical cleaning only, regardless of the rinse involved. Moreover, plaque collected 6 h after rinsing with antimicrobial mouthrinses contained a surplus of antimicrobial activity. Only Listerine showed decreased viability in saliva, but none of the mouthrinses showed any residual antimicrobial activity in saliva. The findings indicate that plaque left behind after mechanical cleaning contributes to the prolonged substantivity of antimicrobial mouthrinses.
本研究旨在确定牙菌斑和唾液对三种抗菌漱口水（李施德林®、美乐家®、佳洁士®）的延长活性（也称为持续性）的贡献，这些漱口水与一种牙膏（Prodent Coolmint®）联合使用。志愿者用不含抗菌成分的牙膏刷牙 4 周，而在最后 2 周，一半志愿者在刷牙的同时使用抗菌漱口水。在实验期末，在口腔卫生后 6 小时收集牙菌斑和唾液样本，并测定细菌浓度和活力。通过仅用机械清洁获得的牙菌斑与在用抗菌漱口水额外使用后的牙菌斑和唾液结合，评估牙菌斑和唾液对持续性的贡献。随后，测定结合牙菌斑的活力。在用漱口水额外冲洗后的牙菌斑样本的活力低于仅用机械清洁获得的牙菌斑，无论使用哪种漱口水都是如此。此外，在用抗菌漱口水冲洗 6 小时后收集的牙菌斑中含有过量的抗菌活性。只有李施德林®显示在唾液中的活力降低，但没有一种漱口水在唾液中显示出任何残留的抗菌活性。研究结果表明，机械清洁后残留的牙菌斑有助于延长抗菌漱口水的持续性。
