Cutillas Pedro R, Timms John F
Analytical Signalling Group, Centre for Cell Signalling, Institute of Cancer, Bart's and the London School of Medicine, Queen Mary University of London, London, UK.
Methods Mol Biol. 2010;658:3-17. doi: 10.1007/978-1-60761-780-8_1.
LC-MS is a powerful technique in biomolecular research. In addition to its uses as a tool for protein and peptide quantization, LC-MS can also be used to quantify the activity of signalling and metabolic pathways in a multiplex and comprehensive manner, i.e. as an 'activitomic' tool. Taking cancer research as an illustrative example of application, this review discusses the concepts of biochemical pathway analysis using LC-MS-based proteomic and activitomic techniques.
液相色谱-质谱联用技术（LC-MS）是生物分子研究中的一项强大技术。除了用作蛋白质和肽定量工具外，LC-MS还可用于以多重和全面的方式量化信号传导和代谢途径的活性，即作为一种“活性组学”工具。以癌症研究作为应用的一个说明性例子，本综述讨论了使用基于LC-MS的蛋白质组学和活性组学技术进行生化途径分析的概念。
