Chalkley Robert
Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, USA.
Methods Mol Biol. 2010;658:47-60. doi: 10.1007/978-1-60761-780-8_3.
Mass spectrometers now have sufficient sensitivity and acquisition rates to allow analysis of complex proteomic samples on a chromatographic timescale. In this chapter the different instrument options for protein and peptide analysis will be presented, along with their relative strengths and weaknesses for producing different types of information, such as protein identification, modification characterization, or reporting quantitative measurements.
质谱仪现在具有足够的灵敏度和采集速率，能够在色谱时间尺度上对复杂的蛋白质组学样品进行分析。在本章中，将介绍用于蛋白质和肽分析的不同仪器选项，以及它们在生成不同类型信息（如蛋白质鉴定、修饰表征或报告定量测量结果）方面的相对优缺点。
