Łastawska Katarzyna
Department of Physical Chemistry, Faculty of Pharmacy Medical University, Warsaw.
Rocz Panstw Zakl Hig. 2010;61(2):151-4.
Seven commercial food supplements present on the Polish market were evaluated for their in vitro antioxidant capacity. The selected products were in the form of hard gelatin capsules. They contained the extracts from chokeberry, cranberry, blueberry and green tea. The mixture of vitamins and minerals as well as the product containing vitamin C in substantial dose were included into comparison. The products were examined using three methods in order to evaluate their antioxidant capacity: electron paramagnetic resonance (EPR), Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbing antioxidant capacity (ORAC) assays. Total polyphenolic content was determined according to Folin-Ciocalteu method The results were calculated per capsule. All studied preparations showed antioxidant properties and may provide substantial antioxidant protection. The in vitro antioxidant capacity varied considerably and was associated with the content of polyphenols in the capsule. The supplement with 250 mg of green tea extract was the most potent antioxidant in all assays. Nevertheless it must be remembered that the amounts of extracts were different in encapsulated products. As the quality of extracts and their properties are miscellaneous, there is a need to standardize dietary antioxidant supplements with respect to their antioxidant capacity if effective doses are to be recommended.
对波兰市场上现有的七种商业食品补充剂的体外抗氧化能力进行了评估。所选产品为硬明胶胶囊形式。它们含有来自黑果腺肋花楸、蔓越莓、蓝莓和绿茶的提取物。将维生素和矿物质混合物以及含有大量维生素C的产品纳入比较。使用三种方法对这些产品进行检测,以评估其抗氧化能力:电子顺磁共振(EPR)、Trolox等效抗氧化能力(TEAC)、氧自由基吸收抗氧化能力(ORAC)测定。根据福林-西奥尔特法测定总多酚含量。结果按每粒胶囊计算。所有研究的制剂均显示出抗氧化特性,并可能提供实质性的抗氧化保护。体外抗氧化能力差异很大,且与胶囊中多酚的含量有关。含有250毫克绿茶提取物的补充剂在所有检测中都是最有效的抗氧化剂。然而,必须记住,胶囊产品中提取物的含量不同。由于提取物的质量及其特性各不相同,如果要推荐有效剂量,就需要在膳食抗氧化补充剂的抗氧化能力方面进行标准化。