Betancor-Fernández Alejandro, Pérez-Gálvez Antonio, Sies Helmut, Stahl Wilhelm
Institut für Biochemie und Molekularbiologie I, Heinrich-Heine-Universität Düsseldorf, Postfach 101007, D-40001 Düsseldorf, Germany.
J Pharm Pharmacol. 2003 Jul;55(7):981-6. doi: 10.1211/0022357021468.
Pharmaceutical preparations derived from natural sources such as vegetables often contain compounds that contribute to the antioxidant defence system and apparently play a role in the protection against degenerative diseases. In the present study, commercial preparations containing extracts of turmeric, artichoke, devil's claw and garlic or salmon oil were investigated. The products were divided into fractions of different polarity, and their antioxidant activity was determined using the Trolox equivalent antioxidant capacity (TEAC) assay. This test is based on the efficacy of the test material to scavenge 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) derived radicals. Total phenols were determined in all fractions as well as specific carotenoids in the most lipophilic fraction to assess their contribution to the antioxidant activity. For comparison, the radical scavenging effect of selected constituents of the extracts such as curcumin, luteolin, kaempferol, chlorogenic acid, harpagoside, beta-carotene and alpha-tocopherol was investigated and compared with that of Trolox. Curcumin, luteolin, kaempferol, chlorogenic acid and beta-carotene showed an antioxidant activity superior to Trolox in the TEAC assay; harpagoside was barely active. All fractions of the turmeric extract preparation exhibited pronounced antioxidant activity, which was assigned to the presence of curcumin and other polyphenols. The antioxidant activity corresponding to the artichoke leaf extract was higher in the aqueous fractions than in the lipophilic fractions. Similarly, devil's claw extract was particularly rich in water-soluble antioxidants. Harpagoside, a major compound in devil's claw, did not contribute significantly to its antioxidant activity. The antioxidant capacity of the garlic preparation was poor in the TEAC assay. That of salmon oil was mainly attributed to vitamin E, which is added to the product for stabilization. In all test preparations, the antioxidant activity was significantly correlated with the content of total phenolic compounds.
源自蔬菜等天然来源的药物制剂通常含有有助于抗氧化防御系统的化合物，并且显然在预防退行性疾病中发挥作用。在本研究中，对含有姜黄、朝鲜蓟、魔鬼爪、大蒜提取物或鲑鱼油的商业制剂进行了研究。将产品分为不同极性的馏分，并使用Trolox等效抗氧化能力（TEAC）测定法测定其抗氧化活性。该测试基于测试材料清除2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）衍生自由基的功效。测定了所有馏分中的总酚以及最亲脂性馏分中的特定类胡萝卜素，以评估它们对抗氧化活性的贡献。为了进行比较，研究了提取物中选定成分如姜黄素、木犀草素、山奈酚、绿原酸、哈帕苷、β-胡萝卜素和α-生育酚的自由基清除作用，并与Trolox进行了比较。在TEAC测定中，姜黄素、木犀草素、山奈酚、绿原酸和β-胡萝卜素表现出优于Trolox的抗氧化活性；哈帕苷几乎没有活性。姜黄提取物制剂的所有馏分均表现出明显的抗氧化活性，这归因于姜黄素和其他多酚的存在。朝鲜蓟叶提取物的抗氧化活性在水相馏分中高于亲脂性馏分。同样，魔鬼爪提取物特别富含水溶性抗氧化剂。魔鬼爪中的主要化合物哈帕苷对其抗氧化活性贡献不大。大蒜制剂在TEAC测定中的抗氧化能力较差。鲑鱼油的抗氧化能力主要归因于添加到产品中用于稳定化的维生素E。在所有测试制剂中，抗氧化活性与总酚类化合物的含量显著相关。
