Blood and Tissue Bank, Granvia de l'Hospitalet, 201, 08908 l'Hospitalet de Llobregat, Barcelona, Spain.
Vox Sang. 2010 Oct;99(3):267-73. doi: 10.1111/j.1423-0410.2010.01341.x.
The infusion of thawed haematopoietic progenitor cells from apheresis (HPC-A) is associated with minor but frequent adverse reactions (ARs), which has been mainly attributed to dimethyl sulphoxide (DMSO). Nevertheless, other factors may play a role in the pathogenesis of such toxicity.
The ARs on a cohort of 423 cryopreserved HPC-A infusions for 398 patients in HPC transplantation program were analysed.
ARs were reported in 105 graft infusions (24·8%) and most of them were graded as mild to moderate. The most frequently reported ARs were gastrointestinal and respiratory, and three patients presented epileptic seizure. The volume of DMSO/kg (P < 0·001), volume of red-blood-cells/kg (P = 0·02), number of nuclear cells (NCs)/kg (P <0·001) and number of granulocytes/kg (P<0·001) in the infused graft were significant in the univariate analysis for the occurrence of ARs. The amount of granulocytes/kg remained significant in the multivariate analysis (P<0·001). The grade of ARs also correlated with the amount of cryopreserved granulocytes.
The incidence and grade of ARs during infusion of cryopreserved HPC-A are related to the amount of granulocytes in the graft.
从单采中输注解冻的造血祖细胞（HPC-A）会引起轻微但频繁的不良反应（ARs），这主要归因于二甲基亚砜（DMSO）。然而，其他因素可能在这种毒性的发病机制中起作用。
对 398 名患者的 HPC 移植计划中 423 次冷冻保存的 HPC-A 输注的队列中的 ARs 进行了分析。
在 105 次移植物输注中报告了 ARs（24.8%），其中大多数为轻度至中度。最常报告的 ARs 是胃肠道和呼吸道，有 3 名患者出现癫痫发作。在输注移植物中 DMSO/kg（P<0.001）、红细胞/kg（P=0.02）、NCs/kg（P<0.001）和粒细胞/kg（P<0.001）的体积在单变量分析中对 ARs 的发生有显著意义。在多变量分析中，粒细胞/kg 的数量仍然具有显著意义（P<0.001）。ARs 的严重程度也与移植物中冷冻保存的粒细胞数量相关。
输注冷冻保存的 HPC-A 期间 ARs 的发生率和严重程度与移植物中粒细胞的数量有关。
