Giri S N, Siegel D M
Department of Veterinary Pharmacology and Toxicology, School of Veterinary Medicine, University of California, Davis 95616.
J Appl Toxicol. 1990 Dec;10(6):429-38. doi: 10.1002/jat.2550100609.
The compound 2-chloro-4-acetotoluidide (CAT) is highly toxic to many avian species, including the starling. In our earlier work, we demonstrated the covalent binding of radioactivity from [14C]-CAT to liver and kidneys of the starling. In the present study, the effects of inducers of mixed-function oxidase (MFO) and non-protein sulfhydryl (NPSH) depletor on the total and covalent binding of [14C]-CAT radioactivity to liver and kidney of the starling were examined. The total and covalently bound radioactivity from [14C]-CAT to liver and kidney were decreased significantly in the starling pretreated with the MFO inducer, 3-methylcholanthrene. However, pretreatment with phenobarbital, another inducer of MFO, had no effect. Pretreatment with the inhibitor of MFO, SKF 525-A, reduced the covalent binding of [14C]-CAT radioactivity to liver but not to kidney. There was no reduction in the NPSH content of liver or kidney following intravenous administration of CAT (3.5 mg kg-1). Reduction of NPSH levels in the liver or kidney following treatment with diethyl maleate caused a significant increase in the covalent binding of [14C]-CAT to kidney but not to liver.
化合物2-氯-4-乙酰甲苯胺（CAT）对包括椋鸟在内的许多鸟类具有高毒性。在我们早期的研究中，我们证明了[14C]-CAT的放射性与椋鸟的肝脏和肾脏发生共价结合。在本研究中，研究了混合功能氧化酶（MFO）诱导剂和非蛋白巯基（NPSH）消耗剂对[14C]-CAT放射性在椋鸟肝脏和肾脏中的总结合及共价结合的影响。用MFO诱导剂3-甲基胆蒽预处理的椋鸟，[14C]-CAT在肝脏和肾脏中的总放射性及共价结合放射性显著降低。然而，用另一种MFO诱导剂苯巴比妥预处理则没有效果。用MFO抑制剂SKF 525-A预处理可降低[14C]-CAT放射性与肝脏的共价结合，但对肾脏无影响。静脉注射CAT（3.5 mg kg-1）后，肝脏或肾脏中的NPSH含量没有降低。用马来酸二乙酯处理后，肝脏或肾脏中NPSH水平的降低导致[14C]-CAT与肾脏的共价结合显著增加，但与肝脏的共价结合没有增加。
