Shi Qin, Wang Xianghui, Yu Wenjuan, Huang Rui
Department of Physics, East China Normal University, Shanghai 200062, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Aug;27(4):875-81.
The proliferation difference between Human Umbilical Vein Endothelial Cells (HUVEC) and Human Umbilical Artery Smooth Muscle Cells (HUASMC) in response to the concentration of silver nanoparticles was investigated via MTT, BCA and FCM tests. The obtained experimental data were statistically analyzed and discussed in order to know the causation of the proliferation difference. The results show there is significant difference in proliferation between HUVEC and HUASMC corresponding to the concentration of silver nanoparticles, and such difference can be attributed to the varied adhesion shape and apoptosis of the cells being influenced by nano-Ag content and Fetal bovine serum (FBS) content in culture medium.
通过MTT、BCA和流式细胞术（FCM）检测，研究了人脐静脉内皮细胞（HUVEC）和人脐动脉平滑肌细胞（HUASMC）在银纳米颗粒浓度作用下的增殖差异。对获得的实验数据进行了统计分析和讨论，以了解增殖差异的原因。结果表明，对应于银纳米颗粒浓度，HUVEC和HUASMC的增殖存在显著差异，这种差异可归因于细胞的黏附形态和凋亡变化，而这些变化受培养基中纳米银含量和胎牛血清（FBS）含量的影响。
