Cui Jian, Zhang Yang-de
Hepatobiliary & Enteric Surgery Research Center, Central South University, Changsha 410000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Jun;27(6):697-9.
To explore the effects of different doses of nano silver of proliferation of cultured vascular endothelial cells in vitro.
The hearts of three newborn SD rats 5 day old were mechanically minced the enzymatically digested with collagenase and trypsin, then vascular endothelial cell were counted, washed and resuspended in Dulbecco's minimumes sential medium (DMEM) added with 20% heat in activated fetal calf serum, then inoculated d in 2% gelatin coated tissue culture flasks. Vascular endothelial cells at passage 3 were used in the experiment. Except for the normal control group, the vascular endothelial cells were cultured with nano silver in various concentrations (0.5, 0.25, 0.125, 0.0625, 0.03125 g/l) for 24 hours, and the morphology and the number of the cultured endothelial cells were observed. Methly thiazolyl tetrazolium (MTT) colorimetry was used to determine the proliferation of the cultured vascular endothelial cells. Flow cytometry (FCM) were used to detect the proliferation index (PI) of the vascular endothelial cells and the expressions of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemical method.
The cell morphology was normal under the inverted microscoped in each group. The number and proliferation activities of vascular endothelial cells were significantly decreased by 0.5, 0.25, 0.125, 0.0625, 0.03125 g/l nano silver compared with those of the blank control group, especially the 0.25 g/l nao silver group, and there were no remarkable changes in with 0.5 g/L, 0.125 g/L, 0.0625 g/L and 0.03125 g/L nano silver groups compared to each other. The same results were seen in the positive rate of PCNA expression and PI.
Nano silver has dose-dependent effects on the proliferation activity of vascular endothelial cells. It inhibited the proliferation of vascular endothelial cells.
探讨不同剂量纳米银对体外培养血管内皮细胞增殖的影响。
取3只5日龄新生SD大鼠心脏，机械剪碎后用胶原酶和胰蛋白酶消化，计数血管内皮细胞，洗涤并重悬于添加20%热灭活胎牛血清的杜尔贝科改良伊格尔培养基（DMEM）中，然后接种于2%明胶包被的组织培养瓶中。实验采用第3代血管内皮细胞。除正常对照组外，血管内皮细胞用不同浓度（0.5、0.25、0.125、0.0625、0.03125 g/L）的纳米银培养24小时，观察培养的内皮细胞形态和数量。采用甲基噻唑基四氮唑（MTT）比色法测定培养的血管内皮细胞增殖情况。采用流式细胞术（FCM）检测血管内皮细胞的增殖指数（PI），并通过免疫组织化学方法检测增殖细胞核抗原（PCNA）的表达。
各组在倒置显微镜下细胞形态正常。与空白对照组相比，0.5、0.25、0.125、0.0625、0.03125 g/L纳米银显著降低了血管内皮细胞的数量和增殖活性，尤其是0.25 g/L纳米银组，而0.5 g/L、0.125 g/L、0.0625 g/L和0.03125 g/L纳米银组之间无明显变化。PCNA表达阳性率和PI也得到相同结果。
纳米银对血管内皮细胞的增殖活性具有剂量依赖性影响。它抑制了血管内皮细胞的增殖。
