MRC Clinical Sciences Centre, Imperial College London, London, UK.
Expert Rev Mol Diagn. 2010 Sep;10(6):777-85. doi: 10.1586/erm.10.62.
With the advent of new antibody engineering technologies, conventional antibodies have been minimized into smaller antibody formats. Small size is an important advantage for current and future diagnostic development. Nanobodies® (Ablynx) are among the smallest known antigen-binding antibody fragments, and are derived from the heavy-chain only antibodies that occur naturally in the serum of Camelidae. Endowed by natural evolution, these Nanobodies inherently exhibit unique biophysical, biochemical and pharmacological characteristics. In addition to their excellent potential as molecules in drug development, Nanobodies possess very attractive functional properties that aid in their development for diagnostic tools. Here we present several examples of currently available applications of Nanobodies to the field of immunosensor for cancer, immunoaffinity chromatography, in vivo and intracellular imaging.
随着新的抗体工程技术的出现，传统抗体已经被简化为更小的抗体形式。小尺寸是当前和未来诊断开发的一个重要优势。纳米抗体（Ablynx）是已知的最小的抗原结合抗体片段之一，源自天然存在于骆驼科血清中的重链抗体。由于自然进化的影响，这些纳米抗体具有独特的物理化学和药理学特性。除了作为药物开发分子的巨大潜力外，纳米抗体还具有非常有吸引力的功能特性，有助于将其开发为诊断工具。在这里，我们介绍了纳米抗体在癌症免疫传感器、免疫亲和色谱、体内和细胞内成像等领域的几种现有应用。
