骆驼科纳米抗体与单链可变片段的全面比较。

Asaadi Yasaman, Jouneghani Fatemeh Fazlollahi, Janani Sara, Rahbarizadeh Fatemeh

Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.

Department of Cell & Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Biomark Res. 2021 Dec 4;9(1):87. doi: 10.1186/s40364-021-00332-6.

By the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains (VHHs) and single-chain variable fragments (scFvs) are the most favored ones. While scFv is used widely in various applications, camelid antibodies (VHHs) can serve as an alternative because of their superior chemical and physical properties such as higher solubility, stability, smaller size, and lower production cost. Here, these two counterparts are compared in structure and properties to identify which one is more suitable for each of their various therapeutic, diagnosis, and research applications.

随着重组DNA技术的出现，人们开发出了许多没有天然免疫球蛋白不良特性的抗体片段。其中，骆驼科动物重链可变区（VHH）和单链可变片段（scFv）是最受青睐的。虽然scFv在各种应用中被广泛使用，但骆驼科动物抗体（VHH）因其优越的化学和物理性质，如更高的溶解度、稳定性、更小的尺寸和更低的生产成本，可作为一种替代选择。在此，对这两种对应物的结构和性质进行比较，以确定哪一种更适合它们各自的各种治疗、诊断和研究应用。