Genomics and Genetics Division, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Proteos, Singapore 138673.
Nat Rev Cancer. 2010 Oct;10(10):696-706. doi: 10.1038/nrc2916. Epub 2010 Sep 16.
Recently, it has become possible to mobilize the Tc1/mariner transposon, Sleeping Beauty (SB), in mouse somatic cells at frequencies high enough to induce cancer. Tumours result from SB insertional mutagenesis of cancer genes, thus facilitating the identification of the genes and signalling pathways that drive tumour formation. A conditional SB transposition system has also been developed that makes it possible to limit where SB mutagenesis occurs, providing a means to selectively model many types of human cancer. SB mutagenesis has already identified a large collection of known cancer genes in addition to a plethora of new candidate cancer genes and potential drug targets.
最近，已经有可能在小鼠体细胞中以足够高的频率动员 Tc1/mariner 转座子、睡美人（SB），从而导致癌症。肿瘤是由 SB 插入突变致癌基因引起的，因此有助于鉴定驱动肿瘤形成的基因和信号通路。还开发了一种条件性 SB 转座系统，使得有可能限制 SB 突变发生的位置，从而提供了选择性模拟多种人类癌症的方法。SB 突变已经鉴定出大量已知的癌症基因，除了大量新的候选癌症基因和潜在的药物靶点。
