State Key Lab of Metal matrix Composites, School of Materials Science & Engineering, Shanghai Jiao Tong University, 800 DongChuan Road, Shanghai, 200240, China.
Analyst. 2010 Nov;135(11):2939-44. doi: 10.1039/c0an00334d. Epub 2010 Sep 15.
A novel approach for selectively encapsulating Cy5 dye molecules via DNA hybridization in cationic phosphorus dendrimers (G(4)(+))/polystyrenesulfonate (PSS) microcapsules has been developed. The hybridization between Cy5 labeled single strand DNA (ssDNA) targets and complementary ssDNA probes was performed in the microcapsules. During hybridization, the ssDNA targets acted as 'vehicles' and Cy5 dye molecules as 'cargos', which led to the selective encapsulation of the Cy5 molecules inside the capsules. The approach may be applied for many small molecules, which are usually difficult to be encapsulated due to their diffusion properties in microcapsules.
一种通过阳离子磷树状大分子（G(4)(+)）/聚苯乙烯磺酸盐（PSS）微胶囊中的 DNA 杂交来选择性包封 Cy5 染料分子的新方法已经被开发出来。Cy5 标记的单链 DNA（ssDNA）靶标与互补 ssDNA 探针在微胶囊中进行杂交。在杂交过程中，ssDNA 靶标作为“载体”，Cy5 染料分子作为“货物”，这导致 Cy5 分子被选择性地包封在胶囊内部。这种方法可以应用于许多小分子，由于它们在微胶囊中的扩散性质，通常难以被包封。
