Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
Molecules. 2013 Jul 17;18(7):8440-60. doi: 10.3390/molecules18078440.
We review the synthesis of dendrimer-containing layer-by-layer (LbL) assemblies and their applications, including biosensing, controlled drug release, and bio-imaging. Dendrimers can be built into LbL films and microcapsules by alternating deposition of dendrimers and counter polymers on the surface of flat substrates and colloidal microparticles through electrostatic bonding, hydrogen bonding, covalent bonding, and biological affinity. Dendrimer-containing LbL assemblies have been used to construct biosensors, in which electron transfer mediators and metal nanoparticles are often coupled with dendrimers. Enzymes have been successfully immobilized on the surface of electrochemical and optical transducers by forming enzyme/dendrimer LbL multilayers. In this way, high-performance enzyme sensors are fabricated. In addition, dendrimer LbL films and microcapsules are useful for constructing drug delivery systems because dendrimers bind drugs to form inclusion complexes or the dendrimer surface is covalently modified with drugs. Magnetic resonance imaging of cancer cells by iron oxide nanoparticles coated with dendrimer LbL film is also discussed.
我们综述了含树枝状大分子的层层自组装的合成及其应用，包括生物传感、控制药物释放和生物成像。通过静电相互作用、氢键、共价键和生物亲和力，将树枝状大分子和抗衡聚合物交替沉积在平坦基底和胶体微球的表面上，可将树枝状大分子构建到层层膜和微胶囊中。含树枝状大分子的层层组装体已被用于构建生物传感器，其中电子转移介体和金属纳米粒子通常与树枝状大分子偶联。通过形成酶/树枝状大分子层层组装，可以将酶成功固定在电化学和光传感器的表面上。通过这种方式，制造出高性能的酶传感器。此外，树枝状大分子的层层膜和微胶囊可用于构建药物输送系统，因为树枝状大分子可将药物结合形成包合物，或者通过树枝状大分子表面与药物进行共价修饰。我们还讨论了用树枝状大分子层层膜包覆的氧化铁纳米粒子对癌细胞的磁共振成像。
