Sansom Institute, University of South Australia, Adelaide, SA, Australia.
Arch Virol. 2010 Nov;155(11):1887-91. doi: 10.1007/s00705-010-0800-3. Epub 2010 Sep 16.
Bacteriophages contribute greatly to bacterial evolution. There has been limited investigation of enterococcal bacteriophages, and only two enterococcal bacteriophages have been sequenced completely. In this study, a novel enterococcal bacteriophage, EFRM31, was isolated from a piggery effluent sample and then characterized. The complete bacteriophage genome was determined by shotgun sequencing. EFRM31 belongs to the family Siphoviridae (order Caudovirales) and has a circular double-stranded DNA genome. The putative EFRM31 genome consists of 16945 nucleotides with a low GC content (34.5%) and does not contain CpG islands. The EFRM31 genome contains 82 putative open reading frames, including 17 with identities to genes required for the assembly of a head-tail bacteriophage and 6 hypothetical proteins of unknown function. In general, the sequencing results from EFRM31 revealed considerable similarity to another enterococcal bacteriophage, EFAP-1. This identity and the order of shared genes suggest a close relationship or a common ancestor for these two bacteriophages.
噬菌体对细菌进化有很大的贡献。肠球菌噬菌体的研究还很有限，只有两种肠球菌噬菌体被完全测序。在这项研究中，从养猪场废水样本中分离到一种新型肠球菌噬菌体 EFRM31，并对其进行了特征描述。通过鸟枪法测序确定了完整的噬菌体基因组。EFRM31 属于肌尾噬菌体科（尾病毒目），具有圆形双链 DNA 基因组。推定的 EFRM31 基因组由 16945 个核苷酸组成，GC 含量低（34.5%），不含 CpG 岛。EFRM31 基因组包含 82 个推定的开放阅读框，其中 17 个与组装头部-尾部噬菌体所需的基因具有同一性，6 个是功能未知的假定蛋白。总的来说，EFRM31 的测序结果与另一种肠球菌噬菌体 EFAP-1 有很大的相似性。这种同一性和共享基因的顺序表明这两种噬菌体之间存在密切的关系或共同的祖先。
