Stevens Roy H, Zhang Hongming, Hsiao Chaiwing, Kachlany Scott, Tinoco Eduardo M B, DePew Jessica, Fouts Derrick E
Laboratory of Oral Infectious Diseases, Temple University Kornberg School of Dentistry, Philadelphia, PA, USA; Department of Endodontics, Temple University Kornberg School of Dentistry, Philadelphia, PA, USA.
Laboratory of Oral Infectious Diseases, Temple University Kornberg School of Dentistry , Philadelphia, PA, USA.
Bacteriophage. 2016 Nov 4;6(4):e1251381. doi: 10.1080/21597081.2016.1251381. eCollection 2016.
ϕEf11, a temperate bacteriophage, was isolated by induction from a root canal isolate of . Sequence analysis suggested that the ϕEf11 genome included a contiguous 8 gene module whose function was related to head structure assembly and another module of 10 contiguous genes whose products were responsible for tail structure assembly. SDS-PAGE analysis of virions of a ϕEf11 derivative revealed 11 well-resolved protein bands. To unify the deduced functional gene assignments emanating from the DNA sequence data, with the structural protein analysis of the purified virus, 6 of the SDS-PAGE bands were subjected to mass spectrometry analysis. 5 of the 6 protein bands analyzed by mass spectrometry displayed identical amino acid sequences to those predicted to be specified by 4 of the ORFs identified in the ϕEf11 genome. These included: ORF8 (predicted scaffold protein), ORF10 (predicted major head protein), ORF15 (predicted major tail protein), and ORF23 (presumptive antireceptor).
温和噬菌体ϕEf11是从一种根管分离株中通过诱导分离得到的。序列分析表明，ϕEf11基因组包含一个由8个连续基因组成的模块，其功能与头部结构组装有关，还有一个由10个连续基因组成的模块，其产物负责尾部结构组装。对ϕEf11衍生物的病毒粒子进行SDS-PAGE分析，显示出11条分辨率良好的蛋白条带。为了将DNA序列数据推导的功能基因分配与纯化病毒的结构蛋白分析统一起来，对6条SDS-PAGE条带进行了质谱分析。通过质谱分析的6条蛋白条带中的5条显示出与ϕEf11基因组中鉴定的4个ORF预测的氨基酸序列相同。这些包括：ORF8（预测的支架蛋白）、ORF10（预测的主要头部蛋白）、ORF15（预测的主要尾部蛋白）和ORF23（推定的抗受体）。
